Perivascular T-cell infiltration leads to sustained pulmonary artery remodeling after endothelial cell damage

Michael J. Cuttica, Thomas Langenickel, Audrey Noguchi, Roberto F. Machado, Mark T. Gladwin, Manfred Boehm*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Pulmonary hypertension is a vascular proliferative disease characterized by pulmonary artery remodeling because of dysregulated endothelial and smooth muscle cell proliferation. Although the role of inflammation in the development of the disease is not well-defined, plexogenic lesions in human disease are characterized by perivascular inflammation composed, in part, of T cells. We explored the role of T-cell infiltration on pulmonary vascular remodeling after endothelial cell damage. We induced endothelial cell damage using monocrotaline and isolated the role of T cells by using Rag1tm1Mom mice and performing adoptive T-cell transfer. We found that monocrotaline causes pulmonary vascular endothelial cell injury followed by a perivascular inflammatory response. The infiltration of inflammatory cells primarily involves CD4+ T cells and leads to the progressive muscularization of small (<30 μm) arterioles. Pulmonary vascular proliferative changes were accompanied by progressive and persistent elevations in right ventricular pressure and right ventricular hypertrophy. Supporting the central role of CD4+ T cells in the inflammatory response, Rag1tm1Mom (Rag1-/-) mice, which are devoid of T and B cells, were protected from the development of vascular injury when exposed to monocrotaline. The introduction of T cells from control mice into Rag1-/- mice reproduced the vascular injury phenotype. These data indicate that after endothelial cell damage, CD4+ T-cell infiltration participates in pulmonary vascular remodeling. This finding suggests that a CD41 T-cell immune responsemaycontribute to the pathogenesis of inflammatory vascular lesions seen in some forms of pulmonary hypertension.

Original languageEnglish (US)
Pages (from-to)62-71
Number of pages10
JournalAmerican journal of respiratory cell and molecular biology
Issue number1
StatePublished - Jul 1 2011


  • CD4+ T cells
  • Inflammation
  • Pulmonary vascular remodeling
  • Rag1 mice

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


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