Peroxiredoxin V contributes to antioxidant defense of lung epithelial cells

Pedro C. Avila; Andrei V. Kropotov; Raisa Krutilina; Anna Krasnodembskay; Nikolay V. Tomilin; Vladimir B. Serikov

doi:10.1007/s00408-007-9066-2

Peroxiredoxin V contributes to antioxidant defense of lung epithelial cells

Pedro C. Avila, Andrei V. Kropotov, Raisa Krutilina, Anna Krasnodembskay, Nikolay V. Tomilin, Vladimir B. Serikov

Medicine, Allergy Division

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The aim of this study was to determine the functional significance of peroxiredoxin V (PRXV) in defense against oxidative stress and changes of its expression in human lung inflammation. We used in vitro cell cultures and retrospective analyses of human sputum samples to perform the study. We found that stable clones of lung epithelial cell lines A549 and U1810 with reduced expression of PRXV were prone to oxidative damage. Upregulation of PRXV decreased induction of DNA double-strand breaks and protein oxidation by cigarette smoke extract and hydrogen peroxide. Transfection with PRXV-carrying plasmid protected Calu-3 confluent epithelial cell sheets from alterations in barrier permeability induced by oxidative stress. In human sputum proinflammatory cytokines, myeloperoxidase, and PRXV were increased during viral-induced inflammation. We conclude that PRXV is an important antioxidant protein of lung epithelial cells. Its expression in the human lung increases in inflammation.

Original language	English (US)
Pages (from-to)	103-114
Number of pages	12
Journal	Lung
Volume	186
Issue number	2
DOIs	https://doi.org/10.1007/s00408-007-9066-2
State	Published - Apr 2008

Keywords

DNA double-strand breaks
Epithelial cells
Oxidation
Peroxiredoxin V

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine

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10.1007/s00408-007-9066-2

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title = "Peroxiredoxin V contributes to antioxidant defense of lung epithelial cells",

abstract = "The aim of this study was to determine the functional significance of peroxiredoxin V (PRXV) in defense against oxidative stress and changes of its expression in human lung inflammation. We used in vitro cell cultures and retrospective analyses of human sputum samples to perform the study. We found that stable clones of lung epithelial cell lines A549 and U1810 with reduced expression of PRXV were prone to oxidative damage. Upregulation of PRXV decreased induction of DNA double-strand breaks and protein oxidation by cigarette smoke extract and hydrogen peroxide. Transfection with PRXV-carrying plasmid protected Calu-3 confluent epithelial cell sheets from alterations in barrier permeability induced by oxidative stress. In human sputum proinflammatory cytokines, myeloperoxidase, and PRXV were increased during viral-induced inflammation. We conclude that PRXV is an important antioxidant protein of lung epithelial cells. Its expression in the human lung increases in inflammation.",

keywords = "DNA double-strand breaks, Epithelial cells, Oxidation, Peroxiredoxin V",

author = "Avila, {Pedro C.} and Kropotov, {Andrei V.} and Raisa Krutilina and Anna Krasnodembskay and Tomilin, {Nikolay V.} and Serikov, {Vladimir B.}",

note = "Funding Information: Research described in this article was supported by Philip Morris USA Inc. and by Philip Morris International, and by INTAS Genomics grant 05-1000004-7755. Clinical samples were collected under funding from NIAID (AI50496 and AI057506). P. Avila is currently funded by the Ernest S. Bazley Grant to Northwestern University. The authors thank Dr. Homer Boushey, Ms. Theresa Ward, and Ms. Jane Liu for their assistance in conducting the clinical common cold study, and Ms. Junqing Shen for technical work with rhinovirus infection of epithelial cell cultures.",

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doi = "10.1007/s00408-007-9066-2",

language = "English (US)",

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AU - Krutilina, Raisa

AU - Krasnodembskay, Anna

AU - Tomilin, Nikolay V.

AU - Serikov, Vladimir B.

N1 - Funding Information: Research described in this article was supported by Philip Morris USA Inc. and by Philip Morris International, and by INTAS Genomics grant 05-1000004-7755. Clinical samples were collected under funding from NIAID (AI50496 and AI057506). P. Avila is currently funded by the Ernest S. Bazley Grant to Northwestern University. The authors thank Dr. Homer Boushey, Ms. Theresa Ward, and Ms. Jane Liu for their assistance in conducting the clinical common cold study, and Ms. Junqing Shen for technical work with rhinovirus infection of epithelial cell cultures.

PY - 2008/4

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N2 - The aim of this study was to determine the functional significance of peroxiredoxin V (PRXV) in defense against oxidative stress and changes of its expression in human lung inflammation. We used in vitro cell cultures and retrospective analyses of human sputum samples to perform the study. We found that stable clones of lung epithelial cell lines A549 and U1810 with reduced expression of PRXV were prone to oxidative damage. Upregulation of PRXV decreased induction of DNA double-strand breaks and protein oxidation by cigarette smoke extract and hydrogen peroxide. Transfection with PRXV-carrying plasmid protected Calu-3 confluent epithelial cell sheets from alterations in barrier permeability induced by oxidative stress. In human sputum proinflammatory cytokines, myeloperoxidase, and PRXV were increased during viral-induced inflammation. We conclude that PRXV is an important antioxidant protein of lung epithelial cells. Its expression in the human lung increases in inflammation.

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Peroxiredoxin V contributes to antioxidant defense of lung epithelial cells

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