Persistent nuclear actin filaments inhibit transcription by RNA polymerase II

Leonid A. Serebryannyy, Megan Parilla, Paolo Annibale, Christina M. Cruz, Kyle Laster, Enrico Gratton, Dmitri Kudryashov, Steven T. Kosak, Cara J. Gottardi, Primal de Lanerolle*

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Actin is abundant in the nucleus and it is clear that nuclear actin has important functions. However, mystery surrounds the absence of classical actin filaments in the nucleus. To address this question, we investigated how polymerizing nuclear actin into persistent nuclear actin filaments affected transcription by RNA polymerase II. Nuclear filaments impaired nuclear actin dynamics by polymerizing and sequestering nuclear actin. Polymerizing actin into stable nuclear filaments disrupted the interaction of actin with RNA polymerase II and correlated with impaired RNA polymerase II localization, dynamics, gene recruitment, and reduced global transcription and cell proliferation. Polymerizing and crosslinking nuclear actin in vitro similarly disrupted the actin-RNA-polymerase-II interaction and inhibited transcription. These data rationalize the general absence of stable actin filaments in mammalian somatic nuclei. They also suggest a dynamic pool of nuclear actin is required for the proper localization and activity of RNA polymerase II.

Original languageEnglish (US)
Pages (from-to)3412-3425
Number of pages14
JournalJournal of cell science
Volume129
Issue number18
DOIs
StatePublished - 2016

Keywords

  • Actin
  • Nuclear actin filaments
  • Nucleus
  • RNA polymerase II
  • Transcription

ASJC Scopus subject areas

  • Cell Biology

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    Serebryannyy, L. A., Parilla, M., Annibale, P., Cruz, C. M., Laster, K., Gratton, E., Kudryashov, D., Kosak, S. T., Gottardi, C. J., & de Lanerolle, P. (2016). Persistent nuclear actin filaments inhibit transcription by RNA polymerase II. Journal of cell science, 129(18), 3412-3425. https://doi.org/10.1242/jcs.195867