Pervasive combinatorial modification of histone H3 in human cells

Benjamin A. Garcia, James J. Pesavento, Craig A. Mizzen*, Neil L. Kelleher

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

206 Scopus citations

Abstract

We developed a platform using hydrophilic interaction chromatography and high-resolution tandem mass spectrometry (MS) for analyses of histone H3 that allows comprehensive characterization of 'histone codes' at the molecular level. We identified over 150 differentially modified forms of histone H3.2 in asynchronously grown and butyrate-treated HeLa cells, revealing pervasive combinatorial modification previously unaccounted for by other techniques and providing a clarified estimate of the molecular diversity of histone H3 in mammals.

Original languageEnglish (US)
Pages (from-to)487-489
Number of pages3
JournalNature Methods
Volume4
Issue number6
DOIs
StatePublished - Jun 2007

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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