TY - JOUR
T1 - Phagokinetic tracks of 3T3 cells
T2 - Parallels between the orientation of track segments and of cellular structures which contain actin or tubulin
AU - Albrecht-Buehler, Guenter
N1 - Funding Information:
I thank Dr. James D. Watson for his continuing encouragement and support. I am grateful to Dr. Keith Burridge (Cold Spring Harbor Laboratory) and Dr. Gerald M. Fuller (University of Texas, Galveston) for their generous gifts of the antibody preparations which were used in this work, and to Dr. William Gordon (Cold Spring Harbor Laboratory) for generous permission to screen his antibody preparations. Robert M. Lancaster provided excellent experimental help, and Ms. Sallie Chait provided photographic assistence. I am grateful for Ms. Madeline Szadkowski’s patient typing of the manuscript. The work was supported by a Cold Spring Harbor Laboratory Cancer Center grant from the National Cancer Institute and by grants from the NSF.
PY - 1977/10
Y1 - 1977/10
N2 - Phagokinetic tracks were used to determine the current direction of migration in 3T3 cells. Comparing this direction with the orientation of actin- or tubulin-containing cellular structures by indirect immunofluorescence, the following results were obtained. First, the main actin-containing bundles were located at the bottom and tail end of 3T3 cells and ran parallel to the current or preceding direction of migration. Second, the 3 μm long rod-like structure (primary cilium), which contains tubulin and which has been observed by other investigators in transmission electron microscopy (Barnes, 1961; Sorokin, 1962; Wheatley, 1969) and in indirect immunofluorescence (Osborn and Weber, 1976), was oriented predominantly parallel to the substrate and to the current movement direction. It seems possible that the primary cilium has a role in the directional control of a migrating 3T3 cell, and that the main actin containing bundles act as substrate-attached rails along which the nucleus and bulk cytoplasm slide during displacement of the cells.
AB - Phagokinetic tracks were used to determine the current direction of migration in 3T3 cells. Comparing this direction with the orientation of actin- or tubulin-containing cellular structures by indirect immunofluorescence, the following results were obtained. First, the main actin-containing bundles were located at the bottom and tail end of 3T3 cells and ran parallel to the current or preceding direction of migration. Second, the 3 μm long rod-like structure (primary cilium), which contains tubulin and which has been observed by other investigators in transmission electron microscopy (Barnes, 1961; Sorokin, 1962; Wheatley, 1969) and in indirect immunofluorescence (Osborn and Weber, 1976), was oriented predominantly parallel to the substrate and to the current movement direction. It seems possible that the primary cilium has a role in the directional control of a migrating 3T3 cell, and that the main actin containing bundles act as substrate-attached rails along which the nucleus and bulk cytoplasm slide during displacement of the cells.
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U2 - 10.1016/0092-8674(77)90109-X
DO - 10.1016/0092-8674(77)90109-X
M3 - Article
C2 - 334371
AN - SCOPUS:0017709729
VL - 12
SP - 333
EP - 339
JO - Cell
JF - Cell
SN - 0092-8674
IS - 2
ER -