Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging

Malorie Blancard; K. Ashley Fetterman; Paul W. Burridge

doi:10.1007/978-1-0716-2573-6_10

Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging

Malorie Blancard, K. Ashley Fetterman, Paul W. Burridge^*

^*Corresponding author for this work

Pharmacology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	241-253
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-0716-2573-6_10
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2547
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

This work was supported by the National Institutes of Health (NIH) Distinguished Scholars Program (DSP) to LMR and the Division of Intramural Research (DIR) of the National Institute on Minority Health and Health Disparities (NIMHD) at NIH (1ZIAMD000016 and 1ZIAMD000018). This work was supported by the FAPA project (PVI0122029) from Universidad de Los Andes, granted to A.V.A.

Keywords

Arrhythmia
Calcium imaging
Drug screening
High-throughput
hiPSC-derived cardiomyocytes

ASJC Scopus subject areas

Genetics
Molecular Biology

Access to Document

10.1007/978-1-0716-2573-6_10

Cite this

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abstract = "Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.",

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Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging. / Blancard, Malorie; Fetterman, K. Ashley; Burridge, Paul W.
Methods in Molecular Biology. Humana Press Inc., 2022. p. 241-253 (Methods in Molecular Biology; Vol. 2547).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AU - Fetterman, K. Ashley

AU - Burridge, Paul W.

N1 - Funding Information: This work was supported by the National Institutes of Health (NIH) Distinguished Scholars Program (DSP) to LMR and the Division of Intramural Research (DIR) of the National Institute on Minority Health and Health Disparities (NIMHD) at NIH (1ZIAMD000016 and 1ZIAMD000018). This work was supported by the FAPA project (PVI0122029) from Universidad de Los Andes, granted to A.V.A. Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

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AB - Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.

KW - Arrhythmia

KW - Calcium imaging

KW - Drug screening

KW - High-throughput

KW - hiPSC-derived cardiomyocytes

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Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging

Abstract

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Keywords

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