Pharmacokinetics of 99mTc(Sn)-and 131I-labeled Anti-Carcinoembryonic Antigen Monoclonal Antibody Fragments in Nude Mice

A Michael Zimmer*, Joanne M. Kazikiewicz, Steven T. Rosen, Stewart M Spies

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The biodistribution, radioimmunoimaging, and high pressure liquid chromatography activity profiles of 99mTc(Sn) and l31I-labeled anti-carcinoembryonic antigen monoclonal antibody fragments were compared. Nude mice, bearing specific (colon carcinoma, LS174T) and nonspecific (pancreatic carcinoma, MIA) xenografts were given injections of the respective radiolabeled antibody fragments and also of irrelevant 125I-labeled antibody fragments (MOPC-21). The animals were imaged at 24 h after being given injections, they were sacrificed, and biodistribution studies were performed. Results of the study showed high kidney uptake [48.6% injected dose (ID)/g ± 8.1% (SD)] and low tumor uptake (1.5% ID/g ± 0.6%) for 99mTc(Sn)-labeled fragments and higher uptake (4.4% ID/g ± 0.6%) for 131I-labeled fragments, resulting in a higher localization index for the radioiodinated monoclonal antibody fragments. Imaging results showed good tumor visualization at 24 h after injection for the 131I-labeled fragments and poor tumor visualization with predominant kidney uptake for 99mTc(Sn)-labeled fragments. After radiolalbeling, high pressure liquid chromatography analysis indicated that I31I was primarily associated with F(ab')2fragments, whereas 99mTc was mostly associated with Fab' fragments.

Original languageEnglish (US)
Pages (from-to)1691-1694
Number of pages4
JournalCancer Research
Volume47
Issue number6
StatePublished - Mar 15 1987

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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