Phase separation in binary mixtures of bipolar and monopolar lipid dispersions revealed by ²H NMR spectroscopy, small angle x-ray scattering, and molecular theory

Binary mixtures of C₂₀BAS and POPC membranes were studied by solid-state ²H NMR spectroscopy and small angle x-ray scattering (SAXS) over a wide range of concentrations and at different temperatures. Three specifically deuterated C₂₀BAS derivatives-[1′,1′, 20′,20′-²H₄]C₂₀BAS, [2′,2′,19′,19′-²H₄]C ₂₀BAS, and [10′,11′-²H₂]C ₂₀BAS - combined with protiated 1-palmitoyl-2-oleoyl-sn-glycero-3- phosphocholine (POPC), as well as membranes containing POPC-d₃₁ and fully protiated bolalipid, were used in NMR experiments to obtain structural information for the mixtures. The ²H NMR spectra of [10′,11′-²H₂]C₂₀BAS/POPC membrane dispersions reveal that the bolalipid is predominantly in the transmembrane conformation at high bolalipid concentrations (100, 90, and 70 mol %). At ≤50 mol % C₂₀BAS, smaller quadrupolar couplings appear in the spectra, indicating the presence of U-shaped conformers. The proportion of U-shaped bolalipids increases as the amount of POPC in the membrane increases; however, the transmembrane component remains the dominant bolalipid conformation in the membrane even at 45°C and 10 mol % C₂₀BAS, where it accounts for ∼50% of the bolalipid population. The large fraction of C₂₀BAS transmembrane conformers, regardless of the C₂₀BAS/POPC ratio, together with the findings from molecular mean-field theory calculations, suggests the coexistence of phase-separated bolalipid-rich domains and POPC-rich domains. A single lamellar repeat distance was observed in SAXS experiments corresponding to the average repeat spacing expected for C₂₀BAS-and POPC-rich domains. These observations are consistent with the presence of microphase-separated domains in the mixed membrane samples that arise from POPC-C₂₀BAS hydrophobic mismatch.