Phasic Dopamine Signals in the Nucleus Accumbens that Cause Active Avoidance Require Endocannabinoid Mobilization in the Midbrain

Jennifer M. Wenzel, Erik B. Oleson, Willard N. Gove, Anthony B. Cole, Utsav Gyawali, Hannah M. Dantrassy, Rebecca J. Bluett, Dilyan I. Dryanovski, Garret D. Stuber, Karl Deisseroth, Brian N. Mathur, Sachin Patel, Carl R. Lupica, Joseph F. Cheer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Phasic dopamine (DA) release accompanies approach toward appetitive cues. However, a role for DA in the active avoidance of negative events remains undetermined. Warning signals informing footshock avoidance are associated with accumbal DA release, whereas depression of DA is observed with unavoidable footshock. Here, we reveal a causal role of phasic DA in active avoidance learning; specifically, optogenetic activation of DA neurons facilitates avoidance, whereas optical inhibition of these cells attenuates it. Furthermore, stimulation of DA neurons during presentation of a fear-conditioned cue accelerates the extinction of a passive defensive behavior (i.e., freezing). Dopaminergic control of avoidance requires endocannabinoids (eCBs), as perturbing eCB signaling in the midbrain disrupts avoidance, which is rescued by optical stimulation of DA neurons. Interestingly, once the avoidance task is learned, neither DA nor eCB manipulations affect performance, suggesting that once acquisition occurs, expression of this behavior is subserved by other anatomical frameworks. Our findings establish an instrumental role for DA release in learning active responses to aversive stimuli and its control by eCB signaling. Wenzel et al. demonstrate that phasic mesolimbic dopamine promotes behavior motivated by a cue that predicts a negative event. This dopamine signal is controlled by midbrain endocannabinoids. However, once this behavior is well learned, it becomes independent of these systems.

Original languageEnglish (US)
Pages (from-to)1392-1404.e5
JournalCurrent Biology
Volume28
Issue number9
DOIs
StatePublished - May 7 2018
Externally publishedYes

Keywords

  • avoidance
  • dopamine
  • endocannabinoids
  • fear
  • footshock
  • negative reinforcement
  • nucleus accumbens
  • prefrontal cortex
  • rat
  • rimonabant

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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