Phenethyl isothiocyanate inhibits nitrosamine carcinogenesis in a model for study of oral cancer chemoprevention

Dennis B. Solt; Kuo Wei Chang; Irene Helenowski; Alfred W. Rademaker

doi:10.1016/j.canlet.2003.08.021

Phenethyl isothiocyanate inhibits nitrosamine carcinogenesis in a model for study of oral cancer chemoprevention

Dennis B. Solt^*, Kuo Wei Chang, Irene Helenowski, Alfred W. Rademaker

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The anticarcinogenic effect of phenethyl isothiocyanate (PEITC) was examined in hamster buccal pouch mucosa exposed to N-nitrosomethylbenzylamine (NMBA). Unscheduled DNA synthesis (UDS) and induction of intraepithelial γ-glutamyl transpeptidase (γ-GT) histochemical foci were assessed as potential predictors of anticarcinogenicity. UDS mediated by in vitro exposure to NMBA (at 10 and 50 mM) was examined in mucosal samples derived following topical exposure of pouch mucosa to PEITC at concentrations of 0.5, 5, or 50 mM. In vivo PEITC pretreatment reduced NMBA-induced UDS in a dose dependent manner. PEITC treatment reduced induction of γ-GT foci, detected in epithelial wholemounts derived over a period of 8-13 weeks of NMBA application, by an average of 96%. PEITC also reduced tumor formation by 94%. γ-GT, in particular, may be a useful indicator for identification of effective oral cancer chemopreventive agents and combinations of agents.

Original language	English (US)
Pages (from-to)	147-152
Number of pages	6
Journal	Cancer Letters
Volume	202
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2003.08.021
State	Published - Dec 30 2003

Keywords

Chemoprevention
N- nitrosomethylbenzylamine
Oral cancer
Phenethyl isothiocyanate
Unscheduled DNA synthesis
γ-Glutamyl transpeptidase

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2003.08.021

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title = "Phenethyl isothiocyanate inhibits nitrosamine carcinogenesis in a model for study of oral cancer chemoprevention",

abstract = "The anticarcinogenic effect of phenethyl isothiocyanate (PEITC) was examined in hamster buccal pouch mucosa exposed to N-nitrosomethylbenzylamine (NMBA). Unscheduled DNA synthesis (UDS) and induction of intraepithelial γ-glutamyl transpeptidase (γ-GT) histochemical foci were assessed as potential predictors of anticarcinogenicity. UDS mediated by in vitro exposure to NMBA (at 10 and 50 mM) was examined in mucosal samples derived following topical exposure of pouch mucosa to PEITC at concentrations of 0.5, 5, or 50 mM. In vivo PEITC pretreatment reduced NMBA-induced UDS in a dose dependent manner. PEITC treatment reduced induction of γ-GT foci, detected in epithelial wholemounts derived over a period of 8-13 weeks of NMBA application, by an average of 96%. PEITC also reduced tumor formation by 94%. γ-GT, in particular, may be a useful indicator for identification of effective oral cancer chemopreventive agents and combinations of agents.",

keywords = "Chemoprevention, N- nitrosomethylbenzylamine, Oral cancer, Phenethyl isothiocyanate, Unscheduled DNA synthesis, γ-Glutamyl transpeptidase",

author = "Solt, {Dennis B.} and Chang, {Kuo Wei} and Irene Helenowski and Rademaker, {Alfred W.}",

note = "Funding Information: This research is supported by US Department of Health and Human Services, NCI Grant 1 RO3 CA89788-01A1.",

year = "2003",

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doi = "10.1016/j.canlet.2003.08.021",

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AU - Solt, Dennis B.

AU - Chang, Kuo Wei

AU - Helenowski, Irene

AU - Rademaker, Alfred W.

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PY - 2003/12/30

Y1 - 2003/12/30

N2 - The anticarcinogenic effect of phenethyl isothiocyanate (PEITC) was examined in hamster buccal pouch mucosa exposed to N-nitrosomethylbenzylamine (NMBA). Unscheduled DNA synthesis (UDS) and induction of intraepithelial γ-glutamyl transpeptidase (γ-GT) histochemical foci were assessed as potential predictors of anticarcinogenicity. UDS mediated by in vitro exposure to NMBA (at 10 and 50 mM) was examined in mucosal samples derived following topical exposure of pouch mucosa to PEITC at concentrations of 0.5, 5, or 50 mM. In vivo PEITC pretreatment reduced NMBA-induced UDS in a dose dependent manner. PEITC treatment reduced induction of γ-GT foci, detected in epithelial wholemounts derived over a period of 8-13 weeks of NMBA application, by an average of 96%. PEITC also reduced tumor formation by 94%. γ-GT, in particular, may be a useful indicator for identification of effective oral cancer chemopreventive agents and combinations of agents.

AB - The anticarcinogenic effect of phenethyl isothiocyanate (PEITC) was examined in hamster buccal pouch mucosa exposed to N-nitrosomethylbenzylamine (NMBA). Unscheduled DNA synthesis (UDS) and induction of intraepithelial γ-glutamyl transpeptidase (γ-GT) histochemical foci were assessed as potential predictors of anticarcinogenicity. UDS mediated by in vitro exposure to NMBA (at 10 and 50 mM) was examined in mucosal samples derived following topical exposure of pouch mucosa to PEITC at concentrations of 0.5, 5, or 50 mM. In vivo PEITC pretreatment reduced NMBA-induced UDS in a dose dependent manner. PEITC treatment reduced induction of γ-GT foci, detected in epithelial wholemounts derived over a period of 8-13 weeks of NMBA application, by an average of 96%. PEITC also reduced tumor formation by 94%. γ-GT, in particular, may be a useful indicator for identification of effective oral cancer chemopreventive agents and combinations of agents.

KW - Chemoprevention

KW - N- nitrosomethylbenzylamine

KW - Oral cancer

KW - Phenethyl isothiocyanate

KW - Unscheduled DNA synthesis

KW - γ-Glutamyl transpeptidase

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Phenethyl isothiocyanate inhibits nitrosamine carcinogenesis in a model for study of oral cancer chemoprevention

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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