Phenethyl isothiocyanate(PEIT) inhibits nitrosamine-mediated carcinogenesis in hamster buccal pouch epithelium(HBPE)

Z. Batrouni*, K. W. Chang, D. Solt

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to determine whether PEIT, a vegetable-derived cancer chemopreventive agent inhibits HBPE carcinogenesis mediated by N-methyl-N-benzylnitrosamine(M-BN). PEIT(50mM) in corn oil(CO) was topically applied to both buccal pouches of three hamsters(group 1), thrice-weekly for 2 weeks followed by twice-weekly(Monday/Thursday) topical treatments of a mixture of MBN and PEIT(both at 50mM) for 22 weeks. During the 22 weeks of MBN treatment, the group 1 hamsters also received PEIT(50mM) in CO, on Tuesdays and Fridays. Three group 2 hamsters were similarly treated with the vehicle CO, and MBN in CO, during the 24 week experiment. At the completion of the experiment, whereas the group 2(unprotected) hamsters exhibited a total of 60 epithelial neoplasms(23 ± 10 tumors/hamster), including squamous cell carcinomas, a total of only 4 epithelial neoplasms were observed in the group 1(PEIT protected) hamsters pouches. In a follow-up experiment, PEIT(25mM) in CO also strongly inhibited MBN-mediated induction of microscopic epithelial cell foci exhibiting either gamma-glutamyl transpeptidase(GGT) histochemical activity or immunohistochemical staining for the p53 tumor suppressor gene product. These presumptive precancerous foci were identified in HBPE whole mounts prepared after 8 to 13 weeks of topical MBN(25mM) treatment. We conclude that PEIT is a potent inhibitor of MBN-mediated HBPE carcinogenesis, and that early GGT and p53 foci are predictive of chemoprevention in this experimental model of oral carcinogenesis.

Original languageEnglish (US)
Number of pages1
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume82
Issue number2
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

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