Phenotype of lymphocytes mediating copolymer-specific humoral immunity in ethanol-consuming C57BL/6 mice

Carl Waltenbaugh*, Linda Hsiung

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Little is known about the mechanisms of impaired immune function in alcoholic patients. We have previously shown that ethanol consumption by mice alters copolymer-specific humoral and cellular immune responses. Does ethanol consumption eliminate suppressor T cells, allowing nonresponder mice to make humoral immune responses to poly(Glu50Tyr50) (GT)? Female C57BL/6 mice were fed a nutritionally complete liquid diet containing 35% ethanol-derived calories for up to 33 days. Control mice were fed an isocaloric control liquid diet or remained on a solid diet and water. Mice fed the ethanol-containing diet made GT-specific plaque-forming cell (PFC) responses, whereas mice fed liquid control or solid diets did not. Lymphocytes from ethanol liquid diet-consuming mice helped splenocytes from either solid or liquid control diet mice to make a GT-specific PFC response. The cells mediating help were nylon wool nonadherent, CD4-bearing T cells. These findings suggest that ethanol does not eliminate copolymer-specific suppressor cells, but instead alters the functional capability of helper T cells for humoral immune responses.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalAlcohol
Volume11
Issue number1
DOIs
StatePublished - Jan 1 1994

Keywords

  • Alcohol ingestion
  • In vitro immunization
  • Lymphocyte subsets
  • Mouse model
  • Synthetic copolymer antigens

ASJC Scopus subject areas

  • Health(social science)
  • Biochemistry
  • Toxicology
  • Neurology
  • Behavioral Neuroscience

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