Phenotypic alteration in retroviral gene expression by leukemia-resistant thymocytes differentiating in leukemia-susceptible recipients

Syamal K. Datta; Samuel D. Waksal; Robert S. Schwartz

doi:10.1016/0092-8674(80)90398-0

Phenotypic alteration in retroviral gene expression by leukemia-resistant thymocytes differentiating in leukemia-susceptible recipients

Syamal K. Datta^*, Samuel D. Waksal, Robert S. Schwartz

^*Corresponding author for this work

Medicine, Rheumatology Division

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

(AKR × NZB)F₁ mice possess the dominant genes, Akv-1, Akv-2, Nzv-1^a and Nzv-2^a, which determine the expression of ecotropic and xenotropic viruses. Nevertheless, their thymic lymphocytes fail to produce these agents, and these mice are resistant to leukemia. We investigated the mechanism of this cell-specific restriction in radiation chimeras. (AKR × NZB)F₁ thymocytes that had differentiated in lethally irradiated AKR recipients produced high levels of ecotropic and xenotropic viruses and showed marked amplification of MuLV antigen expression. Polytropic viruses could also be isolated from such thymocytes. These virological changes in chimeric thymocytes were donor- and host-specific and occurred only when (AKR × NZB)F₁ bone marrow cells were inoculated into AKR recipients. This inductive capacity of the host environment could be detected in irradiated AKR recipients as early as age 2 months. The phenotypic changes brought about in leukemia-resistant (AKR × NZB)F₁ thymocytes by the leukemia-susceptible AKR thymic microenvironment may be the result of a three-component inductive system.

Original language	English (US)
Pages (from-to)	171-179
Number of pages	9
Journal	Cell
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(80)90398-0
State	Published - Jan 1980

Funding

We acknowledge the expert technical assistance of Isaac Robert Mendelson. Ann Marie Champ, Norma Robert and Rhonda Sinoff and wish to thank Marrian Gleason and Elaine McGlame for typing the manuscript. This work was supported by two National Cancer Institute grantstoS.K.D.;onetoS.D.W.;and,twotoR.S.S.S.K.D.isaFaculty Research Awardee of the American Cancer Society. S.D.W. is a recipient of a Scholar Award from the Leukemia Society of America. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

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General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(80)90398-0

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@article{a1731fd9d5b449b6977d0b6c585f879e,

title = "Phenotypic alteration in retroviral gene expression by leukemia-resistant thymocytes differentiating in leukemia-susceptible recipients",

abstract = "(AKR × NZB)F1 mice possess the dominant genes, Akv-1, Akv-2, Nzv-1a and Nzv-2a, which determine the expression of ecotropic and xenotropic viruses. Nevertheless, their thymic lymphocytes fail to produce these agents, and these mice are resistant to leukemia. We investigated the mechanism of this cell-specific restriction in radiation chimeras. (AKR × NZB)F1 thymocytes that had differentiated in lethally irradiated AKR recipients produced high levels of ecotropic and xenotropic viruses and showed marked amplification of MuLV antigen expression. Polytropic viruses could also be isolated from such thymocytes. These virological changes in chimeric thymocytes were donor- and host-specific and occurred only when (AKR × NZB)F1 bone marrow cells were inoculated into AKR recipients. This inductive capacity of the host environment could be detected in irradiated AKR recipients as early as age 2 months. The phenotypic changes brought about in leukemia-resistant (AKR × NZB)F1 thymocytes by the leukemia-susceptible AKR thymic microenvironment may be the result of a three-component inductive system.",

author = "Datta, \{Syamal K.\} and Waksal, \{Samuel D.\} and Schwartz, \{Robert S.\}",

year = "1980",

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doi = "10.1016/0092-8674(80)90398-0",

language = "English (US)",

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T1 - Phenotypic alteration in retroviral gene expression by leukemia-resistant thymocytes differentiating in leukemia-susceptible recipients

AU - Datta, Syamal K.

AU - Waksal, Samuel D.

AU - Schwartz, Robert S.

PY - 1980/1

Y1 - 1980/1

N2 - (AKR × NZB)F1 mice possess the dominant genes, Akv-1, Akv-2, Nzv-1a and Nzv-2a, which determine the expression of ecotropic and xenotropic viruses. Nevertheless, their thymic lymphocytes fail to produce these agents, and these mice are resistant to leukemia. We investigated the mechanism of this cell-specific restriction in radiation chimeras. (AKR × NZB)F1 thymocytes that had differentiated in lethally irradiated AKR recipients produced high levels of ecotropic and xenotropic viruses and showed marked amplification of MuLV antigen expression. Polytropic viruses could also be isolated from such thymocytes. These virological changes in chimeric thymocytes were donor- and host-specific and occurred only when (AKR × NZB)F1 bone marrow cells were inoculated into AKR recipients. This inductive capacity of the host environment could be detected in irradiated AKR recipients as early as age 2 months. The phenotypic changes brought about in leukemia-resistant (AKR × NZB)F1 thymocytes by the leukemia-susceptible AKR thymic microenvironment may be the result of a three-component inductive system.

AB - (AKR × NZB)F1 mice possess the dominant genes, Akv-1, Akv-2, Nzv-1a and Nzv-2a, which determine the expression of ecotropic and xenotropic viruses. Nevertheless, their thymic lymphocytes fail to produce these agents, and these mice are resistant to leukemia. We investigated the mechanism of this cell-specific restriction in radiation chimeras. (AKR × NZB)F1 thymocytes that had differentiated in lethally irradiated AKR recipients produced high levels of ecotropic and xenotropic viruses and showed marked amplification of MuLV antigen expression. Polytropic viruses could also be isolated from such thymocytes. These virological changes in chimeric thymocytes were donor- and host-specific and occurred only when (AKR × NZB)F1 bone marrow cells were inoculated into AKR recipients. This inductive capacity of the host environment could be detected in irradiated AKR recipients as early as age 2 months. The phenotypic changes brought about in leukemia-resistant (AKR × NZB)F1 thymocytes by the leukemia-susceptible AKR thymic microenvironment may be the result of a three-component inductive system.

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AN - SCOPUS:0018838585

SN - 0092-8674

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Phenotypic alteration in retroviral gene expression by leukemia-resistant thymocytes differentiating in leukemia-susceptible recipients

Abstract

Funding

ASJC Scopus subject areas

UN SDGs

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