Phorbol ester-associated changes in ganglioside metabolism

J. D. Burczak; J. R. Moskal; J. E. Trosko; J. L. Fairley; C. C. Sweeley

doi:10.1016/0014-4827(83)90210-0

Phorbol ester-associated changes in ganglioside metabolism

J. D. Burczak, J. R. Moskal, J. E. Trosko, J. L. Fairley, C. C. Sweeley^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of phorbol esters on ganglioside metabolism in contact-inhibited Chinese hamster V79 cells was examined. Three phorbol esters of varying structure and tumor-promoting activity were used. Treatment of cells with tumor-promoting phorbol esters resulted in accumulation of gangliosides and increased incorporation of [1-¹⁴C]palmitate and [9-³H]sialic acid into gangliosides. Moreover, the phorbol esters were found to increase the activity of CMP-sialic acid: lactosylceramide sialyltransferase, the enzyme catalysing the first step in ganglioside biosynthesis. The magnitude of phorbol ester effects on V79 cell ganglioside metabolism correlated with the in vivo phorbol ester tumor-promoting activity.

Original language	English (US)
Pages (from-to)	281-286
Number of pages	6
Journal	Experimental Cell Research
Volume	147
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(83)90210-0
State	Published - Sep 1983

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Cell Biology

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@article{b05ef24774e14d0b8fe1f17f00c81bde,

title = "Phorbol ester-associated changes in ganglioside metabolism",

abstract = "The effect of phorbol esters on ganglioside metabolism in contact-inhibited Chinese hamster V79 cells was examined. Three phorbol esters of varying structure and tumor-promoting activity were used. Treatment of cells with tumor-promoting phorbol esters resulted in accumulation of gangliosides and increased incorporation of [1-14C]palmitate and [9-3H]sialic acid into gangliosides. Moreover, the phorbol esters were found to increase the activity of CMP-sialic acid: lactosylceramide sialyltransferase, the enzyme catalysing the first step in ganglioside biosynthesis. The magnitude of phorbol ester effects on V79 cell ganglioside metabolism correlated with the in vivo phorbol ester tumor-promoting activity.",

author = "Burczak, {J. D.} and Moskal, {J. R.} and Trosko, {J. E.} and Fairley, {J. L.} and Sweeley, {C. C.}",

note = "Funding Information: This work was supported by grants from the NC1 (CA 21104) and the Environmental Agency to J. E. T. and from the NIH (AM 12434) to C. C. S. and J. L. F.",

year = "1983",

month = sep,

doi = "10.1016/0014-4827(83)90210-0",

language = "English (US)",

volume = "147",

pages = "281--286",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Phorbol ester-associated changes in ganglioside metabolism

AU - Burczak, J. D.

AU - Moskal, J. R.

AU - Trosko, J. E.

AU - Fairley, J. L.

AU - Sweeley, C. C.

N1 - Funding Information: This work was supported by grants from the NC1 (CA 21104) and the Environmental Agency to J. E. T. and from the NIH (AM 12434) to C. C. S. and J. L. F.

PY - 1983/9

Y1 - 1983/9

N2 - The effect of phorbol esters on ganglioside metabolism in contact-inhibited Chinese hamster V79 cells was examined. Three phorbol esters of varying structure and tumor-promoting activity were used. Treatment of cells with tumor-promoting phorbol esters resulted in accumulation of gangliosides and increased incorporation of [1-14C]palmitate and [9-3H]sialic acid into gangliosides. Moreover, the phorbol esters were found to increase the activity of CMP-sialic acid: lactosylceramide sialyltransferase, the enzyme catalysing the first step in ganglioside biosynthesis. The magnitude of phorbol ester effects on V79 cell ganglioside metabolism correlated with the in vivo phorbol ester tumor-promoting activity.

AB - The effect of phorbol esters on ganglioside metabolism in contact-inhibited Chinese hamster V79 cells was examined. Three phorbol esters of varying structure and tumor-promoting activity were used. Treatment of cells with tumor-promoting phorbol esters resulted in accumulation of gangliosides and increased incorporation of [1-14C]palmitate and [9-3H]sialic acid into gangliosides. Moreover, the phorbol esters were found to increase the activity of CMP-sialic acid: lactosylceramide sialyltransferase, the enzyme catalysing the first step in ganglioside biosynthesis. The magnitude of phorbol ester effects on V79 cell ganglioside metabolism correlated with the in vivo phorbol ester tumor-promoting activity.

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U2 - 10.1016/0014-4827(83)90210-0

DO - 10.1016/0014-4827(83)90210-0

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VL - 147

SP - 281

EP - 286

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -

Phorbol ester-associated changes in ganglioside metabolism

Abstract

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