Phorbol ester-associated changes in ganglioside metabolism

J. D. Burczak, J. R. Moskal, J. E. Trosko, J. L. Fairley, C. C. Sweeley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The effect of phorbol esters on ganglioside metabolism in contact-inhibited Chinese hamster V79 cells was examined. Three phorbol esters of varying structure and tumor-promoting activity were used. Treatment of cells with tumor-promoting phorbol esters resulted in accumulation of gangliosides and increased incorporation of [1-14C]palmitate and [9-3H]sialic acid into gangliosides. Moreover, the phorbol esters were found to increase the activity of CMP-sialic acid: lactosylceramide sialyltransferase, the enzyme catalysing the first step in ganglioside biosynthesis. The magnitude of phorbol ester effects on V79 cell ganglioside metabolism correlated with the in vivo phorbol ester tumor-promoting activity.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalExperimental Cell Research
Volume147
Issue number2
DOIs
StatePublished - Sep 1983

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Phorbol ester-associated changes in ganglioside metabolism'. Together they form a unique fingerprint.

Cite this