Phospholipases C and the pathogenesis of Listeria.

D. A. Portnoy*, G. A. Smith, H. Goldfine

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Listeria monocytogenes is a model intracellular pathogen which escapes from a host cell vacuole, grows intracytoplasmically, and spreads cell to cell without an extracellular phase. A number of genes necessary for pathogenicity have been discovered, two of which encode phospholipases C, a PI-PLC and a broad-range PLC. Single and double mutants were constructed with in-frame deletions in one or both PLCs. Characterization of the strains indicated that the two PLCs may have overlapping function as the double mutant was 500-fold less virulent while the single mutants had a negligible effect on virulence. The role of the PLCs appears to be multifactorial as PI-PLC has a role in escaping from the initial host vacuole and the broad-range PLC appears to have a role in cell to cell spreading.

Original languageEnglish (US)
Pages (from-to)357-361
Number of pages5
JournalBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]
Volume27
Issue number2
StatePublished - Feb 1994

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

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