Abstract
In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.
Original language | English (US) |
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Pages (from-to) | 65-68 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 130 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2 1991 |
Funding
Supported by MH-25281 to A.R. The authors wish to express their gratitude to Sheila Walker, Ward Virts and Nancy Winter for assistance in the preparation of this manuscript. David Morgan, Ph.D., provided helpfull comments during preparation of this manuscript.
Keywords
- Back-titration
- Depolarization
- Hippocampal slice
- Protein phosphorylation
- Pyruvate dehydrogenase complex
ASJC Scopus subject areas
- General Neuroscience