Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization

Scott T. Cain*, Aryeh Routtenberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalNeuroscience Letters
Volume130
Issue number1
DOIs
StatePublished - Sep 2 1991

Funding

Supported by MH-25281 to A.R. The authors wish to express their gratitude to Sheila Walker, Ward Virts and Nancy Winter for assistance in the preparation of this manuscript. David Morgan, Ph.D., provided helpfull comments during preparation of this manuscript.

Keywords

  • Back-titration
  • Depolarization
  • Hippocampal slice
  • Protein phosphorylation
  • Pyruvate dehydrogenase complex

ASJC Scopus subject areas

  • General Neuroscience

Fingerprint

Dive into the research topics of 'Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization'. Together they form a unique fingerprint.

Cite this