In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Sep 2 1991|
- Hippocampal slice
- Protein phosphorylation
- Pyruvate dehydrogenase complex
ASJC Scopus subject areas