Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization

Scott T. Cain; Aryeh Routtenberg

doi:10.1016/0304-3940(91)90228-L

Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization

Scott T. Cain^*, Aryeh Routtenberg

^*Corresponding author for this work

Psychology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.

Original language	English (US)
Pages (from-to)	65-68
Number of pages	4
Journal	Neuroscience Letters
Volume	130
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90228-L
State	Published - Sep 2 1991

Keywords

Back-titration
Depolarization
Hippocampal slice
Protein phosphorylation
Pyruvate dehydrogenase complex

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(91)90228-L

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title = "Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization",

abstract = "In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.",

keywords = "Back-titration, Depolarization, Hippocampal slice, Protein phosphorylation, Pyruvate dehydrogenase complex",

author = "Cain, {Scott T.} and Aryeh Routtenberg",

note = "Funding Information: Supported by MH-25281 to A.R. The authors wish to express their gratitude to Sheila Walker, Ward Virts and Nancy Winter for assistance in the preparation of this manuscript. David Morgan, Ph.D., provided helpfull comments during preparation of this manuscript.",

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T1 - Phosphorylation of pyruvate dehydrogenase in the hippocampal slice

T2 - Time course of response to cellular depolarization

AU - Cain, Scott T.

AU - Routtenberg, Aryeh

N1 - Funding Information: Supported by MH-25281 to A.R. The authors wish to express their gratitude to Sheila Walker, Ward Virts and Nancy Winter for assistance in the preparation of this manuscript. David Morgan, Ph.D., provided helpfull comments during preparation of this manuscript.

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Y1 - 1991/9/2

N2 - In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.

AB - In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.

KW - Back-titration

KW - Depolarization

KW - Hippocampal slice

KW - Protein phosphorylation

KW - Pyruvate dehydrogenase complex

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Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization

Abstract

Keywords

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