Phosphorylation of pyruvate dehydrogenase in the hippocampal slice: Time course of response to cellular depolarization

Scott T. Cain*, Aryeh Routtenberg

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

In the rat hippocampal slice, we evaluated the effects of potassium depolarization on phosphorylation of the alpha subunit of the pyruvate dehydrogenase complex (E1α, mol. wt.∼41 kDa) using an in vitro back-titration assay. The in vitro back-titration phosphorylation of E1α was maximal following 30 s of depolarization and remained elevated for up to 3 min following termination of the depolarizing pulse. Possible significance of and mechanisms for the depolarization-induced changes in E1α phosphorylation are discussed.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalNeuroscience Letters
Volume130
Issue number1
DOIs
StatePublished - Sep 2 1991

Keywords

  • Back-titration
  • Depolarization
  • Hippocampal slice
  • Protein phosphorylation
  • Pyruvate dehydrogenase complex

ASJC Scopus subject areas

  • Neuroscience(all)

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