Photoactivatable drugs for nicotinic optopharmacology

Sambashiva Banala, Matthew C. Arvin, Nicholas M. Bannon, Xiao Tao Jin, John J. Macklin, Yong Wang, Can Peng, Guiqing Zhao, John J. Marshall, Kyle R. Gee, David L. Wokosin, Veronica J. Kim, J. Michael McIntosh, Anis Contractor, Henry A. Lester, Yevgenia Kozorovitskiy, Ryan M. Drenan*, Luke D. Lavis

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Photoactivatable pharmacological agents have revolutionized neuroscience, but the palette of available compounds is limited. We describe a general method for caging tertiary amines by using a stable quaternary ammonium linkage that elicits a red shift in the activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncageable via one- or two-photon excitation, that is useful to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotemporal scales.

Original languageEnglish (US)
Pages (from-to)347-350
Number of pages4
JournalNature Methods
Volume15
Issue number5
DOIs
StatePublished - Apr 27 2018

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Banala, S., Arvin, M. C., Bannon, N. M., Jin, X. T., Macklin, J. J., Wang, Y., Peng, C., Zhao, G., Marshall, J. J., Gee, K. R., Wokosin, D. L., Kim, V. J., McIntosh, J. M., Contractor, A., Lester, H. A., Kozorovitskiy, Y., Drenan, R. M., & Lavis, L. D. (2018). Photoactivatable drugs for nicotinic optopharmacology. Nature Methods, 15(5), 347-350. https://doi.org/10.1038/nmeth.4637