Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins

Shengwang Zhou, Kevin J. Metcalf, Pradeep Bugga, Jennifer Grant, Milan Mrksich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


This article describes a photochemical approach for independently patterning multiple proteins to an inert substrate, particularly for studies of cell adhesion. A photoactivatable chloropyrimidine ligand was employed for covalent immobilization of SnapTag fusion proteins on self-assembled monolayers of alkanethiolates on gold. A two-step procedure was used: first, patterned UV illumination of the surface activated protein capture ligands, and second, incubation with a SnapTag fusion protein bound to the surface in illuminated regions. Two different fluorescent proteins were patterned in registry with features of 400 nm in size over a 1 mm 2 area. An example is given wherein an anti-carcinoembryonic antigen (anti-CEA) scFv antibody was patterned to direct the selective attachment of a human cancer cell line that express the CEA antigen. This method enables the preparation of surfaces with control over the density and activity of independently patterned proteins.

Original languageEnglish (US)
Pages (from-to)40452-40459
Number of pages8
JournalACS Applied Materials and Interfaces
Issue number47
StatePublished - Nov 28 2018


  • immobilization
  • monolayers
  • photochemistry
  • proteins
  • surface chemistry

ASJC Scopus subject areas

  • Materials Science(all)


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