Photochemical Barcodes

Sicheng Tang, Yang Zhang, Pravat Dhakal, Laura Ravelo, Cheyenne L. Anderson, Kevin M. Collins, Françisco M. Raymo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


A photochemical strategy to encode fluorescence signals in vivo with spatial control was designed around the unique properties of a photoactivatable borondipyrromethene (BODIPY). The photoinduced disconnection of two oxazines, flanking a single BODIPY, in two consecutive steps produces a mixture of three emissive molecules with resolved fluorescence inside polymer beads. The relative amounts and emission intensities of the three fluorophores can be regulated precisely in each bead by adjusting the dose of activating photons to mark individual particles with distinct codes of fluorescence signals. The visible wavelengths and mild illumination sufficient to induce these transformations permit the photochemical barcoding of beads also in living nematodes. Different regions of the same animal can be labeled with distinct barcodes to allow the monitoring of their dynamics for long times with no toxic effects. Thus, our photochemical strategy for the generation of fluorescence barcodes can produce multiple and distinguishable labels in the same biological sample to enable the spatiotemporal tracking of, otherwise indistinguishable, targets.

Original languageEnglish (US)
Pages (from-to)4485-4488
Number of pages4
JournalJournal of the American Chemical Society
Issue number13
StatePublished - Apr 4 2018

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry


Dive into the research topics of 'Photochemical Barcodes'. Together they form a unique fingerprint.

Cite this