Photocrosslinking of 5-iodouracil-substituted RNA and DNA to proteins

Michael C. Willis, Brian J. Hicke, Olke C. Uhlenbeck, Thomas R. Cech, Tad H. Koch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

169 Scopus citations

Abstract

5-Iodouracil-substituted RNA and DNA were crosslinked regiospecifically to associated proteins in yields of 70 to 94% of bound nucleic acid. Irradiation of the iodouracil chromophore with monochromatic, long-wavelength ultraviolet radiation (325 nanometers) eliminates excitation of other nucleic acid and protein chromophores. The combination of high crosslinking yields, excellent specificity, and elimination of photodamage to other chromophores represents an important advance toward the precise identification of contacts in nucleoprotein complexes.

Original languageEnglish (US)
Pages (from-to)1255-1257
Number of pages3
JournalScience
Volume262
Issue number5137
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • General

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