Picomole assay for Nτ-methylhistidine by gas chromatography-Mass spectrometry

D. E. Matthews*, J. B. Starren, A. J. Drexler, D. M. Kipnis, D. M. Bier

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


A simple, sensitive method for measuring Nτ-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation, a single analysis can be completed in 3 min; analysis in duplicate, including sample preparation for 40 samples, can be completed at a rate of 15 min per sample. Nanomole amounts of Nτ-mmethylhistidine in urine or plasma samples are determined with a precision of 0.5%. Picomole amounts, released during in vitro rat epitrochlaris muscle incubations, are measured with a precision of 10%.

Original languageEnglish (US)
Pages (from-to)308-317
Number of pages10
JournalAnalytical Biochemistry
Issue number2
StatePublished - Jan 15 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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