Abstract
A simple, sensitive method for measuring Nτ-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation, a single analysis can be completed in 3 min; analysis in duplicate, including sample preparation for 40 samples, can be completed at a rate of 15 min per sample. Nanomole amounts of Nτ-mmethylhistidine in urine or plasma samples are determined with a precision of 0.5%. Picomole amounts, released during in vitro rat epitrochlaris muscle incubations, are measured with a precision of 10%.
Original language | English (US) |
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Pages (from-to) | 308-317 |
Number of pages | 10 |
Journal | Analytical Biochemistry |
Volume | 110 |
Issue number | 2 |
DOIs | |
State | Published - Jan 15 1981 |
Funding
The authors gratefully acknowledge the technical assistance of B. J. Thielan, L. L. Su-Chu, and H. L. Christopherson. This work was supported in part by National Institutes of Health Grants AM-25994, HD-10667, and RR-00954, the National Livestock and Meat Board, and the National Foundation-March of Dimes.
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology