Picomole assay for Nτ-methylhistidine by gas chromatography-Mass spectrometry

D. E. Matthews; J. B. Starren; A. J. Drexler; D. M. Kipnis; D. M. Bier

doi:10.1016/0003-2697(81)90196-2

Picomole assay for N^τ-methylhistidine by gas chromatography-Mass spectrometry

D. E. Matthews^*, J. B. Starren, A. J. Drexler, D. M. Kipnis, D. M. Bier

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A simple, sensitive method for measuring N^τ-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation, a single analysis can be completed in 3 min; analysis in duplicate, including sample preparation for 40 samples, can be completed at a rate of 15 min per sample. Nanomole amounts of N^τ-mmethylhistidine in urine or plasma samples are determined with a precision of 0.5%. Picomole amounts, released during in vitro rat epitrochlaris muscle incubations, are measured with a precision of 10%.

Original language	English (US)
Pages (from-to)	308-317
Number of pages	10
Journal	Analytical Biochemistry
Volume	110
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(81)90196-2
State	Published - Jan 15 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(81)90196-2

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title = "Picomole assay for Nτ-methylhistidine by gas chromatography-Mass spectrometry",

abstract = "A simple, sensitive method for measuring Nτ-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation, a single analysis can be completed in 3 min; analysis in duplicate, including sample preparation for 40 samples, can be completed at a rate of 15 min per sample. Nanomole amounts of Nτ-mmethylhistidine in urine or plasma samples are determined with a precision of 0.5%. Picomole amounts, released during in vitro rat epitrochlaris muscle incubations, are measured with a precision of 10%.",

author = "Matthews, {D. E.} and Starren, {J. B.} and Drexler, {A. J.} and Kipnis, {D. M.} and Bier, {D. M.}",

note = "Funding Information: The authors gratefully acknowledge the technical assistance of B. J. Thielan, L. L. Su-Chu, and H. L. Christopherson. This work was supported in part by National Institutes of Health Grants AM-25994, HD-10667, and RR-00954, the National Livestock and Meat Board, and the National Foundation-March of Dimes.",

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AU - Matthews, D. E.

AU - Starren, J. B.

AU - Drexler, A. J.

AU - Kipnis, D. M.

AU - Bier, D. M.

N1 - Funding Information: The authors gratefully acknowledge the technical assistance of B. J. Thielan, L. L. Su-Chu, and H. L. Christopherson. This work was supported in part by National Institutes of Health Grants AM-25994, HD-10667, and RR-00954, the National Livestock and Meat Board, and the National Foundation-March of Dimes.

PY - 1981/1/15

Y1 - 1981/1/15

N2 - A simple, sensitive method for measuring Nτ-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation, a single analysis can be completed in 3 min; analysis in duplicate, including sample preparation for 40 samples, can be completed at a rate of 15 min per sample. Nanomole amounts of Nτ-mmethylhistidine in urine or plasma samples are determined with a precision of 0.5%. Picomole amounts, released during in vitro rat epitrochlaris muscle incubations, are measured with a precision of 10%.

AB - A simple, sensitive method for measuring Nτ-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation, a single analysis can be completed in 3 min; analysis in duplicate, including sample preparation for 40 samples, can be completed at a rate of 15 min per sample. Nanomole amounts of Nτ-mmethylhistidine in urine or plasma samples are determined with a precision of 0.5%. Picomole amounts, released during in vitro rat epitrochlaris muscle incubations, are measured with a precision of 10%.

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Picomole assay for N^τ-methylhistidine by gas chromatography-Mass spectrometry

Abstract

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