Abstract
Fluorescence lifetimes and anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are reported. The anisotropy data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84 (1986) 510). A persistence length of about 7 to 10 residues is obtained for the mobilities in the two hormones. The theory is able to account for chain length and probe location effects, but the calculated time dependence of the anisotropy does not fit the experimental curve well at short times.
Original language | English (US) |
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Pages (from-to) | 55-61 |
Number of pages | 7 |
Journal | Chemical Physics Letters |
Volume | 139 |
Issue number | 1 |
DOIs | |
State | Published - Aug 14 1987 |
Funding
This work was supportedb y a grant from the National ScienceF oundation.W e thank Professor Karl Freed for muchh elpfula dvice.J WP was sup-portedb y an NSF IndustrializedC ountriesp ostdoc-toral fellowship,a n INSERM pasteo range,a nd a fellowshipf rom La Fondation pour la Recherche Mtdicale.
ASJC Scopus subject areas
- General Physics and Astronomy
- Physical and Theoretical Chemistry