Fluorescence lifetimes and anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are reported. The anisotropy data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84 (1986) 510). A persistence length of about 7 to 10 residues is obtained for the mobilities in the two hormones. The theory is able to account for chain length and probe location effects, but the calculated time dependence of the anisotropy does not fit the experimental curve well at short times.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Physical and Theoretical Chemistry