Picosecond fluorescence studies of polypeptide dynamics: fluorescence anisotropies and lifetimes

Lin X Q Chen, Jacob W. Petrich*, Graham R. Fleming, Angelo Perico

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Fluorescence lifetimes and anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are reported. The anisotropy data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84 (1986) 510). A persistence length of about 7 to 10 residues is obtained for the mobilities in the two hormones. The theory is able to account for chain length and probe location effects, but the calculated time dependence of the anisotropy does not fit the experimental curve well at short times.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalChemical Physics Letters
Volume139
Issue number1
DOIs
StatePublished - Aug 14 1987

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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