TY - JOUR
T1 - Picosecond fluorescence studies of polypeptide dynamics
T2 - fluorescence anisotropies and lifetimes
AU - Chen, Lin X Q
AU - Petrich, Jacob W.
AU - Fleming, Graham R.
AU - Perico, Angelo
N1 - Funding Information:
This work was supportedb y a grant from the National ScienceF oundation.W e thank Professor Karl Freed for muchh elpfula dvice.J WP was sup-portedb y an NSF IndustrializedC ountriesp ostdoc-toral fellowship,a n INSERM pasteo range,a nd a fellowshipf rom La Fondation pour la Recherche Mtdicale.
PY - 1987/8/14
Y1 - 1987/8/14
N2 - Fluorescence lifetimes and anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are reported. The anisotropy data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84 (1986) 510). A persistence length of about 7 to 10 residues is obtained for the mobilities in the two hormones. The theory is able to account for chain length and probe location effects, but the calculated time dependence of the anisotropy does not fit the experimental curve well at short times.
AB - Fluorescence lifetimes and anisotropies for single tryptophan-containing polypeptide hormones ACTH and glucagon, and a series of their fragments are reported. The anisotropy data are discussed in the context of the theory of Perico and Guenza (J. Chem. Phys. 84 (1986) 510). A persistence length of about 7 to 10 residues is obtained for the mobilities in the two hormones. The theory is able to account for chain length and probe location effects, but the calculated time dependence of the anisotropy does not fit the experimental curve well at short times.
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U2 - 10.1016/0009-2614(87)80150-1
DO - 10.1016/0009-2614(87)80150-1
M3 - Article
AN - SCOPUS:0037889340
SN - 0009-2614
VL - 139
SP - 55
EP - 61
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 1
ER -