PLANT-Dx: A molecular diagnostic for point of use detection of plant pathogens

M. Verosloff, J. Chappell, K. L. Perry, J. R. Thompson, J. B. Lucks

Research output: Contribution to journalArticlepeer-review

Abstract

Synthetic biology based diagnostic technologies have improved upon gold standard diagnostic methodologies by decreasing cost, increasing accuracy, and enhancing portability. However there has been little effort in adapting these technologies towards applications related to point-of-use monitoring of plant and crop health. Here, we take a step towards this vision by developing an approach that couples isothermal amplification of specific plant pathogen genomic sequences with customizable synthetic RNA regulators that are designed to trigger the production of a colorimetric output in cell-free gene expression reactions. We demonstrate our system can sense viral derived sequences with high-sensitivity and specificity, and can be utilized to directly detect viruses from infected plant material. Furthermore, we demonstrate that the entire system can operate using only body heat and naked-eye visual analysis of outputs. We anticipate these strategies to be important components of user-friendly and deployable diagnostic systems that can be configured to detect a range of important plant pathogens.

Original languageEnglish (US)
JournalUnknown Journal
DOIs
StatePublished - Dec 17 2018

Keywords

  • Cell free synthetic biology
  • Crop pathogens
  • Isothermal amplification
  • Plant viruses
  • Point of use diagnostics
  • RNA synthetic biology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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