Abstract
Plaques are parasite-mediated regions of clearing in especially two-dimensional arrays of target cells. They are important for parasite isolation, enumeration, and characterization, particularly, of virus and bacteriophages. A related procedure, called spotting, is not 100% equivalent to plaquing since spots can form without parasite replication. Various efforts have been made to mathematically model plaque formation, in many cases treating the course of plaque formation as a reaction-diffusion process. This article describes what plaques are, how they form, and complications associated with their use as a means of especially bacteriophage visualization.
Original language | English (US) |
---|---|
Title of host publication | Brenner's Encyclopedia of Genetics |
Subtitle of host publication | Second Edition |
Publisher | Elsevier Inc |
Pages | 356-357 |
Number of pages | 2 |
ISBN (Electronic) | 9780080961569 |
ISBN (Print) | 9780123749840 |
DOIs | |
State | Published - Feb 27 2013 |
Keywords
- Bacteriophage
- Efficiency of center of infection
- Efficiency of plating
- Infective center
- Plaques
- Reaction-diffusion
- Virus
ASJC Scopus subject areas
- General Agricultural and Biological Sciences
- General Biochemistry, Genetics and Molecular Biology
- General Medicine