Plasma osteopontin in acute liver failure

The Acute Liver Failure Study Group

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Osteopontin (OPN) is a novel phosphoglycoprotein expressed in Kupffer cells that plays a pivotal role in activating natural killer cells, neutrophils and macrophages. Measuring plasma OPN levels in patients with acute liver failure (ALF) might provide insights into OPN function in the setting of massive hepatocyte injury. Methods: OPN levels were measured using a Quantikine® ELISA assay on plasma from 105 consecutive ALF patients enrolled by the US Acute Liver Failure Study Group, as well as controls including 40 with rheumatoid arthritis (RA) and 35 healthy subjects both before, and 1 and 3. days after undergoing spine fusion (SF) surgery as a model for acute inflammation. Results: Median plasma OPN levels across all etiologies of ALF patients were elevated 10- to 30-fold: overall median 1055. ng/mL; range: 33-19,127), when compared to healthy controls (median in pre-SF patients: 41. ng/mL; range 2.6-86.4). RA and SF post op patients had elevated OPN levels (37. ng/mL and 198. ng/mL respectively), well below those of the ALF patients. Median OPN levels were highest in acetaminophen (3603. ng/mL) and ischemia-related ALF (4102. ng/mL) as opposed to viral hepatitis (706. ng/mL), drug-induced liver injury (353. ng/mL) or autoimmune hepatitis (436. ng/mL), correlating with the degree of hepatocellular damage, as reflected by aminotransferase values (. R value: 0.47 for AST, p<. 0.001). Conclusions: OPN levels appeared to correlate with degree of liver necrosis in ALF. Very high levels were associated with hyperacute injury and good outcomes. Whether OPN exerts a protective effect in limiting disease progression in this setting remains uncertain.

Original languageEnglish (US)
Pages (from-to)270-276
Number of pages7
JournalCytokine
Volume73
Issue number2
DOIs
StatePublished - Jun 1 2015

Fingerprint

Osteopontin
Acute Liver Failure
Liver
Plasmas
Spine
Fusion reactions
Rheumatoid Arthritis
Chemical and Drug Induced Liver Injury
Autoimmune Hepatitis
Kupffer Cells
Macrophages
Wounds and Injuries
Acetaminophen
Transaminases
Natural Killer Cells
Surgery
Hepatitis
Disease Progression
Hepatocytes
Assays

Keywords

  • Acute liver failure
  • Cytokine
  • Inflammation
  • Liver necrosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

The Acute Liver Failure Study Group (2015). Plasma osteopontin in acute liver failure. Cytokine, 73(2), 270-276. https://doi.org/10.1016/j.cyto.2015.02.021
The Acute Liver Failure Study Group. / Plasma osteopontin in acute liver failure. In: Cytokine. 2015 ; Vol. 73, No. 2. pp. 270-276.
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abstract = "Background: Osteopontin (OPN) is a novel phosphoglycoprotein expressed in Kupffer cells that plays a pivotal role in activating natural killer cells, neutrophils and macrophages. Measuring plasma OPN levels in patients with acute liver failure (ALF) might provide insights into OPN function in the setting of massive hepatocyte injury. Methods: OPN levels were measured using a Quantikine{\circledR} ELISA assay on plasma from 105 consecutive ALF patients enrolled by the US Acute Liver Failure Study Group, as well as controls including 40 with rheumatoid arthritis (RA) and 35 healthy subjects both before, and 1 and 3. days after undergoing spine fusion (SF) surgery as a model for acute inflammation. Results: Median plasma OPN levels across all etiologies of ALF patients were elevated 10- to 30-fold: overall median 1055. ng/mL; range: 33-19,127), when compared to healthy controls (median in pre-SF patients: 41. ng/mL; range 2.6-86.4). RA and SF post op patients had elevated OPN levels (37. ng/mL and 198. ng/mL respectively), well below those of the ALF patients. Median OPN levels were highest in acetaminophen (3603. ng/mL) and ischemia-related ALF (4102. ng/mL) as opposed to viral hepatitis (706. ng/mL), drug-induced liver injury (353. ng/mL) or autoimmune hepatitis (436. ng/mL), correlating with the degree of hepatocellular damage, as reflected by aminotransferase values (. R value: 0.47 for AST, p<. 0.001). Conclusions: OPN levels appeared to correlate with degree of liver necrosis in ALF. Very high levels were associated with hyperacute injury and good outcomes. Whether OPN exerts a protective effect in limiting disease progression in this setting remains uncertain.",
keywords = "Acute liver failure, Cytokine, Inflammation, Liver necrosis",
author = "{The Acute Liver Failure Study Group} and Praveen Srungaram and Rule, {Jody A.} and Yuan, {He Jun} and Andreas Reimold and Benny Dahl and Corron Sanders and Lee, {William M.} and Larson, {Anne M.} and Iris Liou and Timothy Davern and Oren Fix and Michael Schilsky and Timothy McCashland and Hay, {J. Eileen} and Natalie Murray and Shaikh, {A. Obaid S.} and Andres Blei and Daniel Ganger and Ganger, {Daniel R} and Han, {Steven H.B.} and Robert Fontana and Brendan McGuire and Chung, {Raymond T.} and Alastair Smith and Robert Brown and Jeffrey Crippin and Edwin Harrison and Adrian Reuben and Santiago Munoz and Rajender Reddy and Stravitz, {R. Todd} and Lorenzo Rossaro and Raj Satyanarayana and Tarek Hassanein",
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The Acute Liver Failure Study Group 2015, 'Plasma osteopontin in acute liver failure', Cytokine, vol. 73, no. 2, pp. 270-276. https://doi.org/10.1016/j.cyto.2015.02.021

Plasma osteopontin in acute liver failure. / The Acute Liver Failure Study Group.

In: Cytokine, Vol. 73, No. 2, 01.06.2015, p. 270-276.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Plasma osteopontin in acute liver failure

AU - The Acute Liver Failure Study Group

AU - Srungaram, Praveen

AU - Rule, Jody A.

AU - Yuan, He Jun

AU - Reimold, Andreas

AU - Dahl, Benny

AU - Sanders, Corron

AU - Lee, William M.

AU - Larson, Anne M.

AU - Liou, Iris

AU - Davern, Timothy

AU - Fix, Oren

AU - Schilsky, Michael

AU - McCashland, Timothy

AU - Hay, J. Eileen

AU - Murray, Natalie

AU - Shaikh, A. Obaid S.

AU - Blei, Andres

AU - Ganger, Daniel

AU - Ganger, Daniel R

AU - Han, Steven H.B.

AU - Fontana, Robert

AU - McGuire, Brendan

AU - Chung, Raymond T.

AU - Smith, Alastair

AU - Brown, Robert

AU - Crippin, Jeffrey

AU - Harrison, Edwin

AU - Reuben, Adrian

AU - Munoz, Santiago

AU - Reddy, Rajender

AU - Stravitz, R. Todd

AU - Rossaro, Lorenzo

AU - Satyanarayana, Raj

AU - Hassanein, Tarek

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Background: Osteopontin (OPN) is a novel phosphoglycoprotein expressed in Kupffer cells that plays a pivotal role in activating natural killer cells, neutrophils and macrophages. Measuring plasma OPN levels in patients with acute liver failure (ALF) might provide insights into OPN function in the setting of massive hepatocyte injury. Methods: OPN levels were measured using a Quantikine® ELISA assay on plasma from 105 consecutive ALF patients enrolled by the US Acute Liver Failure Study Group, as well as controls including 40 with rheumatoid arthritis (RA) and 35 healthy subjects both before, and 1 and 3. days after undergoing spine fusion (SF) surgery as a model for acute inflammation. Results: Median plasma OPN levels across all etiologies of ALF patients were elevated 10- to 30-fold: overall median 1055. ng/mL; range: 33-19,127), when compared to healthy controls (median in pre-SF patients: 41. ng/mL; range 2.6-86.4). RA and SF post op patients had elevated OPN levels (37. ng/mL and 198. ng/mL respectively), well below those of the ALF patients. Median OPN levels were highest in acetaminophen (3603. ng/mL) and ischemia-related ALF (4102. ng/mL) as opposed to viral hepatitis (706. ng/mL), drug-induced liver injury (353. ng/mL) or autoimmune hepatitis (436. ng/mL), correlating with the degree of hepatocellular damage, as reflected by aminotransferase values (. R value: 0.47 for AST, p<. 0.001). Conclusions: OPN levels appeared to correlate with degree of liver necrosis in ALF. Very high levels were associated with hyperacute injury and good outcomes. Whether OPN exerts a protective effect in limiting disease progression in this setting remains uncertain.

AB - Background: Osteopontin (OPN) is a novel phosphoglycoprotein expressed in Kupffer cells that plays a pivotal role in activating natural killer cells, neutrophils and macrophages. Measuring plasma OPN levels in patients with acute liver failure (ALF) might provide insights into OPN function in the setting of massive hepatocyte injury. Methods: OPN levels were measured using a Quantikine® ELISA assay on plasma from 105 consecutive ALF patients enrolled by the US Acute Liver Failure Study Group, as well as controls including 40 with rheumatoid arthritis (RA) and 35 healthy subjects both before, and 1 and 3. days after undergoing spine fusion (SF) surgery as a model for acute inflammation. Results: Median plasma OPN levels across all etiologies of ALF patients were elevated 10- to 30-fold: overall median 1055. ng/mL; range: 33-19,127), when compared to healthy controls (median in pre-SF patients: 41. ng/mL; range 2.6-86.4). RA and SF post op patients had elevated OPN levels (37. ng/mL and 198. ng/mL respectively), well below those of the ALF patients. Median OPN levels were highest in acetaminophen (3603. ng/mL) and ischemia-related ALF (4102. ng/mL) as opposed to viral hepatitis (706. ng/mL), drug-induced liver injury (353. ng/mL) or autoimmune hepatitis (436. ng/mL), correlating with the degree of hepatocellular damage, as reflected by aminotransferase values (. R value: 0.47 for AST, p<. 0.001). Conclusions: OPN levels appeared to correlate with degree of liver necrosis in ALF. Very high levels were associated with hyperacute injury and good outcomes. Whether OPN exerts a protective effect in limiting disease progression in this setting remains uncertain.

KW - Acute liver failure

KW - Cytokine

KW - Inflammation

KW - Liver necrosis

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The Acute Liver Failure Study Group. Plasma osteopontin in acute liver failure. Cytokine. 2015 Jun 1;73(2):270-276. https://doi.org/10.1016/j.cyto.2015.02.021