Plasmon-enhanced colorimetric ELISA with single molecule sensitivity

Si Chen; Mikael Svedendahl; Richard P.Van Duyne; Mikael Käll

doi:10.1021/nl2006092

Plasmon-enhanced colorimetric ELISA with single molecule sensitivity

Si Chen, Mikael Svedendahl, Richard P.Van Duyne, Mikael Käll^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

171 Scopus citations

Abstract

Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, λ_max, and makes it possible to detect the presence of only one or a few HRP molecules per particle.

Original language	English (US)
Pages (from-to)	1826-1830
Number of pages	5
Journal	Nano letters
Volume	11
Issue number	4
DOIs	https://doi.org/10.1021/nl2006092
State	Published - Apr 13 2011

Keywords

ELISA
Surface plasmon
biosensor
single molecules
single particles
spectra imaging

ASJC Scopus subject areas

Condensed Matter Physics
Mechanical Engineering
Bioengineering
General Chemistry
General Materials Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/nl2006092

Cite this

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abstract = "Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, λmax, and makes it possible to detect the presence of only one or a few HRP molecules per particle.",

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doi = "10.1021/nl2006092",

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T1 - Plasmon-enhanced colorimetric ELISA with single molecule sensitivity

AU - Chen, Si

AU - Svedendahl, Mikael

AU - Duyne, Richard P.Van

AU - Käll, Mikael

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N2 - Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, λmax, and makes it possible to detect the presence of only one or a few HRP molecules per particle.

AB - Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, λmax, and makes it possible to detect the presence of only one or a few HRP molecules per particle.

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KW - single molecules

KW - single particles

KW - spectra imaging

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Plasmon-enhanced colorimetric ELISA with single molecule sensitivity

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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