PMR of the self-complementary oligoribonucleotide CpCpGpG

D. B. Arter*, G. C. Walker, O. C. Uhlenbeck, P. G. Schmidt

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Hydrogen bond resonances in H2O and carbon-bound base proton peaks in D2O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H2O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.

Original languageEnglish (US)
Pages (from-to)1089-1094
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number4
DOIs
StatePublished - Dec 23 1974

Fingerprint

Oligoribonucleotides
Protons
Freezing
Hydrogen
Melting
Guanosine
Chemical shift
Base Pairing
Hydrogen bonds
Carbon
RNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Arter, D. B. ; Walker, G. C. ; Uhlenbeck, O. C. ; Schmidt, P. G. / PMR of the self-complementary oligoribonucleotide CpCpGpG. In: Biochemical and Biophysical Research Communications. 1974 ; Vol. 61, No. 4. pp. 1089-1094.
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PMR of the self-complementary oligoribonucleotide CpCpGpG. / Arter, D. B.; Walker, G. C.; Uhlenbeck, O. C.; Schmidt, P. G.

In: Biochemical and Biophysical Research Communications, Vol. 61, No. 4, 23.12.1974, p. 1089-1094.

Research output: Contribution to journalArticle

TY - JOUR

T1 - PMR of the self-complementary oligoribonucleotide CpCpGpG

AU - Arter, D. B.

AU - Walker, G. C.

AU - Uhlenbeck, O. C.

AU - Schmidt, P. G.

PY - 1974/12/23

Y1 - 1974/12/23

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AB - Hydrogen bond resonances in H2O and carbon-bound base proton peaks in D2O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H2O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.

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