PMR of the self-complementary oligoribonucleotide CpCpGpG

D. B. Arter; G. C. Walker; O. C. Uhlenbeck; P. G. Schmidt

doi:10.1016/S0006-291X(74)80395-5

PMR of the self-complementary oligoribonucleotide CpCpGpG

D. B. Arter^*, G. C. Walker, O. C. Uhlenbeck, P. G. Schmidt

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Hydrogen bond resonances in H₂O and carbon-bound base proton peaks in D₂O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H₂O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.

Original language	English (US)
Pages (from-to)	1089-1094
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	61
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(74)80395-5
State	Published - Dec 23 1974

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{070121b2b9814beb8970049deb8c7acc,

title = "PMR of the self-complementary oligoribonucleotide CpCpGpG",

abstract = "Hydrogen bond resonances in H2O and carbon-bound base proton peaks in D2O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H2O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.",

author = "Arter, {D. B.} and Walker, {G. C.} and Uhlenbeck, {O. C.} and Schmidt, {P. G.}",

note = "Funding Information: Acknowledgements This work was supported by grants from the National Institutes of Health GM 18038 (PGS) and GM 19059 (OCU). OCU is the recipient of a Career Development Award from the NIH. GCW is a National Research Council of Canada Postdoctoral Fellow and DBA is a National Institutes of Health Predoctoral Trainee (GM 00722).",

year = "1974",

month = dec,

day = "23",

doi = "10.1016/S0006-291X(74)80395-5",

language = "English (US)",

volume = "61",

pages = "1089--1094",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

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TY - JOUR

T1 - PMR of the self-complementary oligoribonucleotide CpCpGpG

AU - Arter, D. B.

AU - Walker, G. C.

AU - Uhlenbeck, O. C.

AU - Schmidt, P. G.

N1 - Funding Information: Acknowledgements This work was supported by grants from the National Institutes of Health GM 18038 (PGS) and GM 19059 (OCU). OCU is the recipient of a Career Development Award from the NIH. GCW is a National Research Council of Canada Postdoctoral Fellow and DBA is a National Institutes of Health Predoctoral Trainee (GM 00722).

PY - 1974/12/23

Y1 - 1974/12/23

N2 - Hydrogen bond resonances in H2O and carbon-bound base proton peaks in D2O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H2O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.

AB - Hydrogen bond resonances in H2O and carbon-bound base proton peaks in D2O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H2O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

PMR of the self-complementary oligoribonucleotide CpCpGpG

Abstract

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