PMR of the self-complementary oligoribonucleotide CpCpGpG

D. B. Arter*, G. C. Walker, O. C. Uhlenbeck, P. G. Schmidt

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Hydrogen bond resonances in H2O and carbon-bound base proton peaks in D2O are used to monitori the melting of the CpCpGpG helical duplex in 1.0 M NaCl. At O° C in H2O the low field spectrum consists of resonances at 13.18 and 12.45 ppm from DSS due to the hydrogen bonded N-1 protons of the guanosines of the exterior and interior base pairs, respectively. These values are consistent with predictions based on ring current effects expected for an RNA-11 helix. The chemical shifts of the H-8 and H-6 protons of both the interior and exterior bases show highly co-operative melting transitions and exhibit the same midpoint of 51 ± 2° C.

Original languageEnglish (US)
Pages (from-to)1089-1094
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number4
DOIs
StatePublished - Dec 23 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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