Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney

Susan E. Quaggin; Gregory B. Vanden Heuvel; Peter Igarashi

doi:10.1016/S0925-4773(97)00201-3

Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney

Susan E. Quaggin, Gregory B. Vanden Heuvel, Peter Igarashi^*

^*Corresponding author for this work

Medicine, Nephrology Division

Research output: Contribution to journal › Article › peer-review

145 Scopus citations

Abstract

Basic-helix-loop-helix (bHLH) proteins are transcriptional regulatory proteins that govern cell fate determination and differentiation in a variety of tissues. We have identified a novel bHLH protein, named Pod-1, that belongs to a recently-described subfamily of bHLH proteins that have essential roles in the embryonic development of mesodermal tissues. In the adult human and mouse, Pod-1 was most highly expressed in the kidney, lung and heart. In developing mouse embryos, Pod-1 was selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine and pancreas. Pod-1 was also expressed in visceral glomerular epithelial cells (podocytes) in the kidney, and its expression coincided with the onset of podocyte differentiation. The expression of Pod- 1 in embryonic kidney explants was inhibited using antisense oligonucleotides. Inhibition of Pod-1 expression resulted in decreased mesenchymal cell condensation around the ureteric bud and a 40% decrease in ureteric branching. Pod-1 is the first tissue-restricted basic-helix-loop- helix protein that has been identified in the developing kidney where it may play a role in the regulation of morphogenetic events.

Original language	English (US)
Pages (from-to)	37-48
Number of pages	12
Journal	Mechanisms of Development
Volume	71
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(97)00201-3
State	Published - Feb 1998

Funding

We thank Kui Li and Sue Ann Mentone for expert technical assistance and Michele Pucci for expert secretarial assistance. We thank Doris Herzlinger for providing the Dolichos biflorus agglutinin staining protocol. We thank Frank Ruddle, Charles Radding, and Sherman Weissman for critically reviewing earlier versions of the manuscript. S.E.Q. is the recipient of a Clinician Scientist Award from the Medical Research Council of Canada. G.B.V. was the recipient of a postdoctoral fellowship from the American Heart Association Connecticut Affiliate. P.I. is an Established Investigator of the American Heart Association. This work was supported by NIH grant RO1 DK45678 to P.I.

Keywords

Antisense oligonucleotides
Branching morphogenesis
Epithelial-mesenchymal interactions
Kidney development
Transcription factor

ASJC Scopus subject areas

Embryology
Developmental Biology

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10.1016/S0925-4773(97)00201-3

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title = "Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney",

abstract = "Basic-helix-loop-helix (bHLH) proteins are transcriptional regulatory proteins that govern cell fate determination and differentiation in a variety of tissues. We have identified a novel bHLH protein, named Pod-1, that belongs to a recently-described subfamily of bHLH proteins that have essential roles in the embryonic development of mesodermal tissues. In the adult human and mouse, Pod-1 was most highly expressed in the kidney, lung and heart. In developing mouse embryos, Pod-1 was selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine and pancreas. Pod-1 was also expressed in visceral glomerular epithelial cells (podocytes) in the kidney, and its expression coincided with the onset of podocyte differentiation. The expression of Pod- 1 in embryonic kidney explants was inhibited using antisense oligonucleotides. Inhibition of Pod-1 expression resulted in decreased mesenchymal cell condensation around the ureteric bud and a 40% decrease in ureteric branching. Pod-1 is the first tissue-restricted basic-helix-loop- helix protein that has been identified in the developing kidney where it may play a role in the regulation of morphogenetic events.",

keywords = "Antisense oligonucleotides, Branching morphogenesis, Epithelial-mesenchymal interactions, Kidney development, Transcription factor",

author = "Quaggin, {Susan E.} and {Vanden Heuvel}, {Gregory B.} and Peter Igarashi",

note = "Funding Information: We thank Kui Li and Sue Ann Mentone for expert technical assistance and Michele Pucci for expert secretarial assistance. We thank Doris Herzlinger for providing the Dolichos biflorus agglutinin staining protocol. We thank Frank Ruddle, Charles Radding, and Sherman Weissman for critically reviewing earlier versions of the manuscript. S.E.Q. is the recipient of a Clinician Scientist Award from the Medical Research Council of Canada. G.B.V. was the recipient of a postdoctoral fellowship from the American Heart Association Connecticut Affiliate. P.I. is an Established Investigator of the American Heart Association. This work was supported by NIH grant RO1 DK45678 to P.I. ",

year = "1998",

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doi = "10.1016/S0925-4773(97)00201-3",

language = "English (US)",

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AU - Vanden Heuvel, Gregory B.

AU - Igarashi, Peter

N1 - Funding Information: We thank Kui Li and Sue Ann Mentone for expert technical assistance and Michele Pucci for expert secretarial assistance. We thank Doris Herzlinger for providing the Dolichos biflorus agglutinin staining protocol. We thank Frank Ruddle, Charles Radding, and Sherman Weissman for critically reviewing earlier versions of the manuscript. S.E.Q. is the recipient of a Clinician Scientist Award from the Medical Research Council of Canada. G.B.V. was the recipient of a postdoctoral fellowship from the American Heart Association Connecticut Affiliate. P.I. is an Established Investigator of the American Heart Association. This work was supported by NIH grant RO1 DK45678 to P.I.

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N2 - Basic-helix-loop-helix (bHLH) proteins are transcriptional regulatory proteins that govern cell fate determination and differentiation in a variety of tissues. We have identified a novel bHLH protein, named Pod-1, that belongs to a recently-described subfamily of bHLH proteins that have essential roles in the embryonic development of mesodermal tissues. In the adult human and mouse, Pod-1 was most highly expressed in the kidney, lung and heart. In developing mouse embryos, Pod-1 was selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine and pancreas. Pod-1 was also expressed in visceral glomerular epithelial cells (podocytes) in the kidney, and its expression coincided with the onset of podocyte differentiation. The expression of Pod- 1 in embryonic kidney explants was inhibited using antisense oligonucleotides. Inhibition of Pod-1 expression resulted in decreased mesenchymal cell condensation around the ureteric bud and a 40% decrease in ureteric branching. Pod-1 is the first tissue-restricted basic-helix-loop- helix protein that has been identified in the developing kidney where it may play a role in the regulation of morphogenetic events.

AB - Basic-helix-loop-helix (bHLH) proteins are transcriptional regulatory proteins that govern cell fate determination and differentiation in a variety of tissues. We have identified a novel bHLH protein, named Pod-1, that belongs to a recently-described subfamily of bHLH proteins that have essential roles in the embryonic development of mesodermal tissues. In the adult human and mouse, Pod-1 was most highly expressed in the kidney, lung and heart. In developing mouse embryos, Pod-1 was selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine and pancreas. Pod-1 was also expressed in visceral glomerular epithelial cells (podocytes) in the kidney, and its expression coincided with the onset of podocyte differentiation. The expression of Pod- 1 in embryonic kidney explants was inhibited using antisense oligonucleotides. Inhibition of Pod-1 expression resulted in decreased mesenchymal cell condensation around the ureteric bud and a 40% decrease in ureteric branching. Pod-1 is the first tissue-restricted basic-helix-loop- helix protein that has been identified in the developing kidney where it may play a role in the regulation of morphogenetic events.

KW - Antisense oligonucleotides

KW - Branching morphogenesis

KW - Epithelial-mesenchymal interactions

KW - Kidney development

KW - Transcription factor

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Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney

Abstract

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Keywords

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