Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

Vadim Backman; Rajan Gurjar; Kamran Badizadegan; Irving Itzkan; Ramachandra R. Dasari; Lev T. Perelman; Michael S. Feld

doi:10.1109/2944.796325

Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

Vadim Backman, Rajan Gurjar, Kamran Badizadegan, Irving Itzkan, Ramachandra R. Dasari, Lev T. Perelman, Michael S. Feld

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

406 Scopus citations

Abstract

We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities to detect and diagnose precancerous changes in human tissues.

Original language	English (US)
Pages (from-to)	1019-1026
Number of pages	8
Journal	IEEE Journal on Selected Topics in Quantum Electronics
Volume	5
Issue number	4
DOIs	https://doi.org/10.1109/2944.796325
State	Published - Jul 1999

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

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10.1109/2944.796325

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title = "Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ",

abstract = "We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities to detect and diagnose precancerous changes in human tissues.",

author = "Vadim Backman and Rajan Gurjar and Kamran Badizadegan and Irving Itzkan and Dasari, {Ramachandra R.} and Perelman, {Lev T.} and Feld, {Michael S.}",

note = "Funding Information: Manuscript received February 16, 1999; revised May 28, 1999. This work was supported by the NIH under Grant P41-RR02594 and Grant R01-CA53717. V. Backman, R. Gurjar, I. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld are with G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139 USA. K. Badizadegan is with the Department of Pathology, Children{\textquoteright}s Hospital, Boston, MA 02115 USA. Publisher Item Identifier S 1077-260X(99)07526-7.",

year = "1999",

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doi = "10.1109/2944.796325",

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volume = "5",

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journal = "IEEE Journal of Selected Topics in Quantum Electronics",

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T1 - Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

AU - Backman, Vadim

AU - Gurjar, Rajan

AU - Badizadegan, Kamran

AU - Itzkan, Irving

AU - Dasari, Ramachandra R.

AU - Perelman, Lev T.

AU - Feld, Michael S.

N1 - Funding Information: Manuscript received February 16, 1999; revised May 28, 1999. This work was supported by the NIH under Grant P41-RR02594 and Grant R01-CA53717. V. Backman, R. Gurjar, I. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld are with G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139 USA. K. Badizadegan is with the Department of Pathology, Children’s Hospital, Boston, MA 02115 USA. Publisher Item Identifier S 1077-260X(99)07526-7.

PY - 1999/7

Y1 - 1999/7

N2 - We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities to detect and diagnose precancerous changes in human tissues.

AB - We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities to detect and diagnose precancerous changes in human tissues.

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ER -

Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

Abstract

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