Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

Vadim Backman, Rajan Gurjar, Kamran Badizadegan, Irving Itzkan, Ramachandra R. Dasari, Lev T. Perelman, Michael S. Feld

Research output: Contribution to journalArticlepeer-review

367 Scopus citations

Abstract

We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities to detect and diagnose precancerous changes in human tissues.

Original languageEnglish (US)
Pages (from-to)1019-1026
Number of pages8
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume5
Issue number4
DOIs
StatePublished - Jul 1999

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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