Pollen-pistil interactions in Eucalyptus calophylla provide no evidence of a selection mechanism for aluminium tolerance

Louise M. Egertonn-Warburt, Brendon J. Griffin, Byron B. Lamont

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Selection for aluminium (Al) tolerance was assessed by studying pollen-pistil interactions in Eucalyptus calophylla trees colonising a 30-year-old abandoned coal mine-site (soil pH 4.3) compared with E. calophylla trees on an adjacent forest-site (soil pH 5.3). Energy-dispersive X-ray micro-analysis of reproductive tissues demonstrated that low levels of Al occurred in the stigma, lower style and unfertilised ovules of forest-site flowers. In contrast, significantly higher levels of Al were detected in all reproductive tissues of mine-site flowers. Al concentrations were higher at the base of the style than in the stigma. Al was also detected in stigmatic exudates of mine-site flowers. Selection for Al tolerance occurred in the anther of mine-site flowers as pollen from mine-site flowers germinated six-fold (15.6%) compared with forest-site pollen (2.6%) at the highest concentration of Al (22 ppm) used. However, the rate of pollen tube growth was not significantly different between mine- and forest-sites at any Al concentration. Tolerance of Al by the mine-site pollen was not shared by the progeny as there was no increase in the survival or growth of mine-site seedlings in mine soils over forest-site seedlings. Controlled pollinations between mine-/forest-site pollen and mine-site pistils demonstrated that there was no significant difference in the number of mine- or forest-site pollen tubes at any level in the style in mine-site pistils. Pollen tube abnormalities principally occurred in mine-site pistils. We concluded that there is no evidence yet for a genetically-based tolerance of Al in E. calophylla on coal mining soils.

Original languageEnglish (US)
Pages (from-to)541-552
Number of pages12
JournalAustralian Journal of Botany
Volume41
Issue number5
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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