Hybridoma concentrations were reduced in shake flask and continuous culture by medical-grade PVC and polyurethane samples. Cell viability was unaffected and nutrient uptake rates were increased. No inhibition was observed for silicone, C-Flex or Teflon samples. The inhibition by PVC could be reduced by conditioning the sample with complete medium. The reduction in final cell yield and growth rate may result from extraction of one or more growth factors from the medium.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology