Polymer biocompatibility-effect on hybridoma growth and metabolism

William M. Miller; Andy A. Lin; Charles R. Wilke; Harvey W. Blanch

doi:10.1007/BF01025202

Polymer biocompatibility-effect on hybridoma growth and metabolism

William M. Miller^*, Andy A. Lin, Charles R. Wilke, Harvey W. Blanch

^*Corresponding author for this work

Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Hybridoma concentrations were reduced in shake flask and continuous culture by medical-grade PVC and polyurethane samples. Cell viability was unaffected and nutrient uptake rates were increased. No inhibition was observed for silicone, C-Flex or Teflon samples. The inhibition by PVC could be reduced by conditioning the sample with complete medium. The reduction in final cell yield and growth rate may result from extraction of one or more growth factors from the medium.

Original language	English (US)
Pages (from-to)	463-468
Number of pages	6
Journal	Biotechnology Letters
Volume	8
Issue number	7
DOIs	https://doi.org/10.1007/BF01025202
State	Published - Jul 1986

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01025202

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title = "Polymer biocompatibility-effect on hybridoma growth and metabolism",

abstract = "Hybridoma concentrations were reduced in shake flask and continuous culture by medical-grade PVC and polyurethane samples. Cell viability was unaffected and nutrient uptake rates were increased. No inhibition was observed for silicone, C-Flex or Teflon samples. The inhibition by PVC could be reduced by conditioning the sample with complete medium. The reduction in final cell yield and growth rate may result from extraction of one or more growth factors from the medium.",

author = "Miller, {William M.} and Lin, {Andy A.} and Wilke, {Charles R.} and Blanch, {Harvey W.}",

year = "1986",

month = jul,

doi = "10.1007/BF01025202",

language = "English (US)",

volume = "8",

pages = "463--468",

journal = "Biotechnology Letters",

issn = "0141-5492",

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number = "7",

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TY - JOUR

T1 - Polymer biocompatibility-effect on hybridoma growth and metabolism

AU - Miller, William M.

AU - Lin, Andy A.

AU - Wilke, Charles R.

AU - Blanch, Harvey W.

PY - 1986/7

Y1 - 1986/7

N2 - Hybridoma concentrations were reduced in shake flask and continuous culture by medical-grade PVC and polyurethane samples. Cell viability was unaffected and nutrient uptake rates were increased. No inhibition was observed for silicone, C-Flex or Teflon samples. The inhibition by PVC could be reduced by conditioning the sample with complete medium. The reduction in final cell yield and growth rate may result from extraction of one or more growth factors from the medium.

AB - Hybridoma concentrations were reduced in shake flask and continuous culture by medical-grade PVC and polyurethane samples. Cell viability was unaffected and nutrient uptake rates were increased. No inhibition was observed for silicone, C-Flex or Teflon samples. The inhibition by PVC could be reduced by conditioning the sample with complete medium. The reduction in final cell yield and growth rate may result from extraction of one or more growth factors from the medium.

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U2 - 10.1007/BF01025202

DO - 10.1007/BF01025202

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 7

ER -

Polymer biocompatibility-effect on hybridoma growth and metabolism

Abstract

ASJC Scopus subject areas

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