Polymerization and fractionation of house dust mite allergen

L. C. Grammer*, M. A. Shaughnessy, L. S. Holmquist, J. J. Pruzansky, R. Patterson

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Glutaraldehyde polymerization and appropriate fractionation of allergens result in significantly decreased allergenicity with retained immunogenicity. In a manner similar to that employed with other allergens, we have prepared monomer dust mite (MDM) and polymerized dust mite (PDM). As with other polymerized allergens such as polymerized ragweed, PDM is more than 2 logs less allergenic than MDM as determined by cutaneous end point titration in sensitive patients. Moreover, PDM and MDM are similarly immunogenic in rabbits. As determined by inhibition studies (with rabbit antisera) PDM is antigenically complete and contains no new epitopes against which antibody was produced. In summary, we have prepared PDM which has the desired characteristics of reduced allergenicity, retained immunogenicity, antigenic completeness, and absence of new antigenic determinants.

Original languageEnglish (US)
Pages (from-to)195-199
Number of pages5
JournalAllergy Proceedings
Volume14
Issue number3
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Immunology and Allergy

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    Grammer, L. C., Shaughnessy, M. A., Holmquist, L. S., Pruzansky, J. J., & Patterson, R. (1993). Polymerization and fractionation of house dust mite allergen. Allergy Proceedings, 14(3), 195-199. https://doi.org/10.2500/108854193778878637