Polyvalent DNA nanoparticle conjugates stabilize nucleic acids

Dwight S. Seferos, Andrew E. Prigodich, David A. Giljohann, Pinal C. Patel, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

381 Scopus citations

Abstract

Polyvalent oligonucleotide gold nanoparticle conjugates have unique fundamental properties including distance-dependent plasmon coupling, enhanced binding affinity, and the ability to enter cells and resist enzymatic degradation. Stability in the presence of enzymes is a key consideration for therapeutic uses; however the manner and mechanism by which such nanoparticles are able to resist enzymatic degradation is unknown. Here, we quantify the enhanced stability of polyvalent gold oligonucleotide nanoparticle conjugates with respect to enzyme- catalyzed hydrolysis of DNA and present evidence that the negatively charged surfaces of the nanoparticles and resultant high local salt concentrations are responsible for enhanced stability.

Original languageEnglish (US)
Pages (from-to)308-311
Number of pages4
JournalNano letters
Volume9
Issue number1
DOIs
StatePublished - Jan 2009

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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