We investigated the yield of total number of motile spermatozoa from oligozoospermic men by pooling two closely spaced sequential ejaculates. Semen characteristics were compared between sequential ejaculates (within a period of 1 to 4 hours) of 18 oligozoospermic males (sperm concentration <20 X 106/mL and total sperm count <40 X 106 in the ejaculate) and a control group of 16 normozoospermic men. Whereas the median total number of motile sperm of normozoospermic males significantly decreased from 70 X 106 in the first ejaculate to 23 X 106 in the second sequential ejaculate, such a decrease was not detected in oligozoospermic males, 3.6 X 106 and 3.1 X 106, respectively. The percent of normozoospermic and oligozoospermic men who demonstrated a decreased (<50%), a comparable (50% to 150%), or an increased (>150%) total motile sperm count in the second ejaculate in comparison with the first ejaculate were 69%, 31%, and 0 versus 39%, 28%, and 33%, respectively. Consequently, pooling of two sequential ejaculates significantly increased the median total number of motile sperm from normozoospermic males by 144% and from oligozoospermic males by 329%, (to 10.2 X 106). We suggest that pooling of two sequential ejaculates from oligozoospermic males is a simple and cost effective method to increase significantly the total number of motile sperm for intrauterine insemination, in vitro fertilization, gamete intrafallopian transfer, or semen cryopreservation.
|Original language||English (US)|
|Number of pages||4|
|Journal||Fertility and Sterility|
|State||Published - Jan 1 1990|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology