Positional cloning of the mouse circadian Clock gene

David P. King, Yaliang Zhao, Ashvin M. Sangoram, Lisa D. Wilsbacher, Minoru Tanaka*, Marina P. Antoch, Thomas D.L. Steeves, Martha Hotz Vitaterna, Jon M. Kornhauser, Phillip L. Lowrey, Fred W. Turek, Joseph S. Takahashi

*Corresponding author for this work

Research output: Contribution to journalArticle

1040 Scopus citations

Abstract

We used positional cloning to identify the circadian Clock gene in mice. Clock is a large transcription unit with 24 exons spanning ~100,000 bp of DNA from which transcript classes of 7.5 and ~10 kb arise. Clock encodes a novel member of the bHLH-PAS family of transcription factors. In the Clock mutant allele, an A→T nucleotide transversion in a splice donor site causes exon skipping and deletion of 51 amino acids in the CLOCK protein. Clock is a unique gene with known circadian function and with features predicting DNA binding, protein dimerization, and activation domains. CLOCK represents the second example of a PAS domain-containing clock protein (besides Drosophila PERIOD), which suggests that this motif may define an evolutionarily conserved feature of the circadian clock mechanism.

Original languageEnglish (US)
Pages (from-to)641-653
Number of pages13
JournalCell
Volume89
Issue number4
DOIs
StatePublished - May 16 1997

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

King, D. P., Zhao, Y., Sangoram, A. M., Wilsbacher, L. D., Tanaka, M., Antoch, M. P., Steeves, T. D. L., Vitaterna, M. H., Kornhauser, J. M., Lowrey, P. L., Turek, F. W., & Takahashi, J. S. (1997). Positional cloning of the mouse circadian Clock gene. Cell, 89(4), 641-653. https://doi.org/10.1016/S0092-8674(00)80245-7