Positional cloning of the mouse circadian Clock gene

David P. King, Yaliang Zhao, Ashvin M. Sangoram, Lisa D Wilsbacher, Minoru Tanaka*, Marina P. Antoch, Thomas D.L. Steeves, Martha Hotz Vitaterna, Jon M. Kornhauser, Phillip L. Lowrey, Fred W Turek, Joseph S. Takahashi

*Corresponding author for this work

Research output: Contribution to journalArticle

1021 Citations (Scopus)

Abstract

We used positional cloning to identify the circadian Clock gene in mice. Clock is a large transcription unit with 24 exons spanning ~100,000 bp of DNA from which transcript classes of 7.5 and ~10 kb arise. Clock encodes a novel member of the bHLH-PAS family of transcription factors. In the Clock mutant allele, an A→T nucleotide transversion in a splice donor site causes exon skipping and deletion of 51 amino acids in the CLOCK protein. Clock is a unique gene with known circadian function and with features predicting DNA binding, protein dimerization, and activation domains. CLOCK represents the second example of a PAS domain-containing clock protein (besides Drosophila PERIOD), which suggests that this motif may define an evolutionarily conserved feature of the circadian clock mechanism.

Original languageEnglish (US)
Pages (from-to)641-653
Number of pages13
JournalCell
Volume89
Issue number4
DOIs
StatePublished - May 16 1997

Fingerprint

Circadian Clocks
Cloning
CLOCK Proteins
Organism Cloning
Clocks
Exons
Genes
Protein Multimerization
RNA Splice Sites
DNA-Binding Proteins
Transcription Factors
Nucleotides
Alleles
Amino Acids
DNA
Dimerization
Transcription
Chemical activation
Drosophila Clk protein

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

King, D. P., Zhao, Y., Sangoram, A. M., Wilsbacher, L. D., Tanaka, M., Antoch, M. P., ... Takahashi, J. S. (1997). Positional cloning of the mouse circadian Clock gene. Cell, 89(4), 641-653. https://doi.org/10.1016/S0092-8674(00)80245-7
King, David P. ; Zhao, Yaliang ; Sangoram, Ashvin M. ; Wilsbacher, Lisa D ; Tanaka, Minoru ; Antoch, Marina P. ; Steeves, Thomas D.L. ; Vitaterna, Martha Hotz ; Kornhauser, Jon M. ; Lowrey, Phillip L. ; Turek, Fred W ; Takahashi, Joseph S. / Positional cloning of the mouse circadian Clock gene. In: Cell. 1997 ; Vol. 89, No. 4. pp. 641-653.
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King, DP, Zhao, Y, Sangoram, AM, Wilsbacher, LD, Tanaka, M, Antoch, MP, Steeves, TDL, Vitaterna, MH, Kornhauser, JM, Lowrey, PL, Turek, FW & Takahashi, JS 1997, 'Positional cloning of the mouse circadian Clock gene', Cell, vol. 89, no. 4, pp. 641-653. https://doi.org/10.1016/S0092-8674(00)80245-7

Positional cloning of the mouse circadian Clock gene. / King, David P.; Zhao, Yaliang; Sangoram, Ashvin M.; Wilsbacher, Lisa D; Tanaka, Minoru; Antoch, Marina P.; Steeves, Thomas D.L.; Vitaterna, Martha Hotz; Kornhauser, Jon M.; Lowrey, Phillip L.; Turek, Fred W; Takahashi, Joseph S.

In: Cell, Vol. 89, No. 4, 16.05.1997, p. 641-653.

Research output: Contribution to journalArticle

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AU - Steeves, Thomas D.L.

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AU - Kornhauser, Jon M.

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AU - Takahashi, Joseph S.

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King DP, Zhao Y, Sangoram AM, Wilsbacher LD, Tanaka M, Antoch MP et al. Positional cloning of the mouse circadian Clock gene. Cell. 1997 May 16;89(4):641-653. https://doi.org/10.1016/S0092-8674(00)80245-7