TY - JOUR
T1 - Positional mapping for amplified DNA sequences on 1q21-q22 in hepatocellular carcinoma indicates candidate genes over-expression
AU - Wong, Nathalie
AU - Chan, Andrew
AU - Lee, Siu Wah
AU - Lam, Ellan
AU - To, Ka Fai
AU - Lai, Paul B.S.
AU - Li, Xiao Nan
AU - Liew, Choong Tsek
AU - Johnson, Philip J.
N1 - Funding Information:
This work was carried out within the Hong Kong Cancer Genetics Research Group that was supported by the Kadoorie Charitable Foundations, the Research Grants Council of the Hong Kong SAR (Ref. No.: CUHK 4044/01M), and a CUHK direct grant. We are also grateful to the Providence Foundation Limited, Hong Kong, for their continuing support.
PY - 2003/3/1
Y1 - 2003/3/1
N2 - Background/Aims: Comparative genomic hybridization analysis on hepatocellular carcinoma (HCC) indicated frequent gains of 1q and an amplicon at 1q21-q22. Current cytogenetic evidences confer much importance on 1q21-q22, where a role in drug resistance, tumor metastasis and shorter patient survival had been implicated. Methods: Using positional mapping by interphase cytogenetics, we investigated the amplicon 1q21-q22 in five HCC cases. Three amplification maxima represented by yeast artificial chromosomes (YACs) 955E11, 876B11 and 945D5 that mapped to regions 1q21.1, 1q21.2 and 1q22, respectively, were indicated. We further investigated candidate genes expression in the mapped YACs by quantitative reverse-transcription-polymerase chain reaction. A panel of genes encoding protein transcripts involved in apoptosis, cell cycle progression, calcium binding and jumping translocation was studied. Results: Among ten HCC cases with the amplicon 1q21-q22 examined, we found a significant gene expression level of JTB, SHC1, CCT3 and COPA in the tumors than the paired adjacent non-malignant liver tissues (P ≤ 0.04). Conclusions: Our interphase findings on 1q21-q22 pinpointed three affected loci between D1S305 and D1S2369. Upregulation of candidate genes identified within these over-represented regions may represent targets in the progression of HCC and may carry prognostic significance.
AB - Background/Aims: Comparative genomic hybridization analysis on hepatocellular carcinoma (HCC) indicated frequent gains of 1q and an amplicon at 1q21-q22. Current cytogenetic evidences confer much importance on 1q21-q22, where a role in drug resistance, tumor metastasis and shorter patient survival had been implicated. Methods: Using positional mapping by interphase cytogenetics, we investigated the amplicon 1q21-q22 in five HCC cases. Three amplification maxima represented by yeast artificial chromosomes (YACs) 955E11, 876B11 and 945D5 that mapped to regions 1q21.1, 1q21.2 and 1q22, respectively, were indicated. We further investigated candidate genes expression in the mapped YACs by quantitative reverse-transcription-polymerase chain reaction. A panel of genes encoding protein transcripts involved in apoptosis, cell cycle progression, calcium binding and jumping translocation was studied. Results: Among ten HCC cases with the amplicon 1q21-q22 examined, we found a significant gene expression level of JTB, SHC1, CCT3 and COPA in the tumors than the paired adjacent non-malignant liver tissues (P ≤ 0.04). Conclusions: Our interphase findings on 1q21-q22 pinpointed three affected loci between D1S305 and D1S2369. Upregulation of candidate genes identified within these over-represented regions may represent targets in the progression of HCC and may carry prognostic significance.
KW - 1q21-q22
KW - Gene expression
KW - Hepatocellular carcinoma
KW - Interphase fluorescent in situ hybridization
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U2 - 10.1016/S0168-8278(02)00412-9
DO - 10.1016/S0168-8278(02)00412-9
M3 - Article
C2 - 12586295
AN - SCOPUS:0037335209
SN - 0168-8278
VL - 38
SP - 298
EP - 306
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 3
ER -