Positive selecting cell type determines the phenotype of MHC class Ib-restricted CD8 + T cells

Hoonsik Cho, Yaw Bediako, Honglin Xu, Hak Jong Choi, Chyung Ru Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Several studies have demonstrated an apparent link between positive selection on hematopoietic cells (HCs) and an "innate"T-cell phenotype. Whereas conventional CD8 + T cells are primarily selected on thymic epithelial cells (TECs) and certain innate T cells are exclusively selected on HCs, MHC class Ib-restricted CD8 + T cells appear to be selected on both TECs and HCs. However, whether TEC- and HC-selected T cells represent distinct lineages or whether the same T-cell precursors have the capacity to be selected on either cell type is unknown. Using an M3-restricted T-cell receptor transgenic mouse model, we demonstrate that not only are MHC class Ib-restricted CD8 + T cells capable of being selected on either cell type but that selecting cell type directly affects the phenotype of the resulting CD8 + T cells. M3-restricted CD8 + T cells selected on HCs acquire a more activated phenotype and possess more potent effector functions than those selected on TECs. Additionally, these two developmental pathways are active in the generation of the natural pool of M3-restricted CD8 + T cells. Our results suggest that these two distinct populations may allow MHC class Ib-restricted CD8 + T cells to occupy different immunological niches playing unique roles in immune responses to infection.

Original languageEnglish (US)
Pages (from-to)13241-13246
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number32
DOIs
StatePublished - Aug 9 2011

Keywords

  • CD8 T cells
  • H2-M3
  • Innate lymphocytes
  • T-cell development

ASJC Scopus subject areas

  • General

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