Abstract
We have previously noted that a specific amino acid sequence could form the second framework region of human, mouse and rabbit immunoglobulin light chains, suggesting that this sequence has been preserved for 80 million years. Through divergent evolution, each species has acquired a different set of framework region sequences; however, these sets still share a few similar or identical amino acid sequences. In the present study, we have identified such sequences for all four framework regions between human and mouse immunoglobulin light and heavy chains. They may be useful in humanizing or reshaping mouse or rat antibodies for therapeutic applications in human patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1141-1146 |
| Number of pages | 6 |
| Journal | Molecular Immunology |
| Volume | 29 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1992 |
Funding
Acknowledgements-Wew ould like to thank Dr T. W. Chang of Tanox BiosystemsI,n c. (Houston, TX, U.S.A.) for helpful discussionsT. his work is aidedb y Grants SROl-AI-125616a nd 3ROl-AI-27508 from the National Institute of Allergy and Infectious Diseases and DBM890-1840 from the National ScienceF oundation to E. A. Kabat of Columbia University; and a grant from the Leukemia Research Foundation of Chicago to T. T. Wu of NorthwesternU niversity. Work with the PROPHET computers ystemi s supportedb y a grant to Columbia University by the National Institute of Allergy and Infectious Diseases, the National Cancer Institute, the National Institute of Diabetes, Digestive, and Kidney Diseases,t he National Institute of General Medical Sciences, and the National Library of Medicine,w ith a subcontractf rom Columbia University to Bolt, Beranek and Newman, Inc. (Cambridge,M A 02138,U .S.A.).
ASJC Scopus subject areas
- Molecular Biology
- Immunology