Postsynaptic element contributes to the delay in synaptogenesis in synapsin I-deficient neurons

Adriana Ferreira*, Lian Li, Lih Shen Chin, Paul Greengard, Kenneth S. Kosik

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In a previous study, we reported a retardation in process outgrowth and synapse formation in cultured hippocampal neurons from synapsin I-deficient mice. Here we investigated whether this delay in synaptogenesis was attributable to pre- or postsynaptic elements. The experimental paradigm used in this study involved the establishment of heterochronic cocultures of neurons from wild-type and synapsin I-deficient mice. Newly cultured axons from wild-type and synapsin I-deficient neurons established synapses with mature wild-type postsynaptic elements after 24 and 72 h, respectively. In contrast, synapsin I-deficient postsynaptic elements were able to receive synapses only after 9 days in culture, representing a 5-day delay compared to controls. The results suggest a broad role for synapsin I in the structural development of the synapse, participating directly or indirectly in the maturation of both presynaptic and postsynaptic sites.

Original languageEnglish (US)
Pages (from-to)286-299
Number of pages14
JournalMolecular and Cellular Neurosciences
Volume8
Issue number4
DOIs
StatePublished - Oct 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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