Potassium channel activators abolish excitotoxicity in cultured hippocampal pyramidal neurons

April E. Abele, Richard J. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

When hippocampal pyramidal neurons are grown in culture they develop excitatory synaptic contacts. If these cultures are perfused with Mg2+-free, glycine supplemented medium the neurons exhibit fluctuations in [Ca2+]i and associated cell death ('excitotoxicity'). These phenomena involve the activation of NMDA receptors. When cultures are treated with the K+-channel activators cromakalim and diazoxide both the [Ca2+]i fluctuations and the neuronal death are abolished. These effects are reversed by the sulfonylurea glyburide. It thus appears that K+-channel activators may be a novel therapeutic intervention in epilepsy and associated disorders.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalNeuroscience Letters
Volume115
Issue number2-3
DOIs
StatePublished - Jul 31 1990

Keywords

  • Calcium
  • Excitotoxicity
  • Glutamate
  • Hippocampus
  • Potassium channel
  • Sulfonylurea

ASJC Scopus subject areas

  • Neuroscience(all)

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