Potent modulation of neuronal nicotinic acetylcholine receptor-channel by ethanol

Keiichi Nagata, Gary L. Aistrup, Chao Sheng Huang, William Marszalec, Jin Ho Song, Jay Z. Yeh, Toshio Narahashi*

*Corresponding author for this work

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Controversies remain over which ion channels are the most sensitive to ethanol. We have found that ethanol potently modulates the neuronal nicotinic acetylcholine receptor-channel at micromolar concentrations with an EC50 of 88.5 μM, which is significantly lower than most values previously reported for other ion channels. Prolonged application of ethanol accelerated the decay phase of acetylcholine-induced currents, caused single-channels to open in bursts, and shortened the mean open time, all of which reflect increased receptor desensitization. However, ethanol slowed the decay phase of the current induced by a brief application of acetylcholine, which may indicate that ethanol manifests its action by causing an increase in the affinity of the receptor for acetylcholine. These results suggest that neuronal nicotinic acetylcholine receptors may be important target sites of ethanol, particularly in the early stages of ethanol intoxication.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalNeuroscience Letters
Volume217
Issue number2-3
DOIs
StatePublished - Oct 18 1996

Keywords

  • Desensitization
  • Ethanol
  • Ion channels
  • Nicotinic acetylcholine receptor
  • PC12 cells

ASJC Scopus subject areas

  • Neuroscience(all)

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