TY - JOUR
T1 - Potential of Human Neural Precursor Cells in Diabetic Retinopathy Therapeutics–Preclinical Model
AU - Saçaki, Claudia Sayuri
AU - Mogharbel, Bassam Felipe
AU - Stricker, Priscila Elias Ferreira
AU - Dziedzic, Dilcele Silva Moreira
AU - Irioda, Ana Carolina
AU - Perussolo, Maiara Carolina
AU - Somma, André Tavares
AU - Montiani-Ferreira, Fabiano
AU - Moreno, Juan Carlos Duque
AU - Dornbusch, Peterson
AU - Sato, Mário
AU - Shiokawa, Naoye
AU - de Noronha, Lúcia
AU - Nagashima, Seigo
AU - Bacellar-Galdino, Marianna
AU - Franco, Célia Regina Cavichiolo
AU - Abdelwahid, Eltyeb
AU - Athayde Teixeira de Carvalho, Katherine
N1 - Funding Information:
This research received no external funding. First author was MSc student of Pequeno Príncipe Faculties with Grant from Coordination for the Improvement of Higher Education Personnel- Brazil (CAPES). Finance code 001. We thank Coordination for the Improvement of Higher Education Personnel- Brazil (CAPES).
Publisher Copyright:
© 2021 Taylor & Francis Group, LLC.
PY - 2022
Y1 - 2022
N2 - Purpose: This study aimed to evaluate a cell therapy strategy with human neural precursor cells (hNPCs) to treat diabetic retinopathy (DR) in Wistar rats induced to diabetes by injecting streptozotocin. Material and Methods: The Wharton’s jelly mesenchymal stem cells (WJ-MSCs) were isolated, expanded, and seeded onto a biopolymer substrate to develop neurospheres and obtain the hNPCs. The animals were divided into three groups: non-diabetic (ND) n = four, diabetic without treatment (DM) n = nine, and diabetic with cell therapy (DM + hNPCs) n = nine. After 8 weeks of diabetes induction and DR characteristics installed, intravitreal injection of hNPCs (1 × 106 cell/µL) was performed in the DM + hNPCs group. Optical Coherence Tomography (OCT) and Electroretinography (ERG) evaluations were conducted before and during diabetes and after cell therapy. Four weeks posttreatment, histopathological and immunohistochemistry analyses were performed. Results: The repair of the retinal structures in the treated group (DM + hNPCs) was observed by increased thickness of neuroretinal layers, especially in the ganglion cell and photoreceptor layers, higher ERG oscillatory potentials (OPs) amplitudes, and transplanted hNPCs integration into the Retinal Pigment Epithelium. Conclusions: The results indicate that hNPCs reduced DR progression by a neuroprotective effect and promoted retinal repair, making them potential candidates for regenerating the neuroretinal tissue.
AB - Purpose: This study aimed to evaluate a cell therapy strategy with human neural precursor cells (hNPCs) to treat diabetic retinopathy (DR) in Wistar rats induced to diabetes by injecting streptozotocin. Material and Methods: The Wharton’s jelly mesenchymal stem cells (WJ-MSCs) were isolated, expanded, and seeded onto a biopolymer substrate to develop neurospheres and obtain the hNPCs. The animals were divided into three groups: non-diabetic (ND) n = four, diabetic without treatment (DM) n = nine, and diabetic with cell therapy (DM + hNPCs) n = nine. After 8 weeks of diabetes induction and DR characteristics installed, intravitreal injection of hNPCs (1 × 106 cell/µL) was performed in the DM + hNPCs group. Optical Coherence Tomography (OCT) and Electroretinography (ERG) evaluations were conducted before and during diabetes and after cell therapy. Four weeks posttreatment, histopathological and immunohistochemistry analyses were performed. Results: The repair of the retinal structures in the treated group (DM + hNPCs) was observed by increased thickness of neuroretinal layers, especially in the ganglion cell and photoreceptor layers, higher ERG oscillatory potentials (OPs) amplitudes, and transplanted hNPCs integration into the Retinal Pigment Epithelium. Conclusions: The results indicate that hNPCs reduced DR progression by a neuroprotective effect and promoted retinal repair, making them potential candidates for regenerating the neuroretinal tissue.
KW - Diabetic retinopathy
KW - cell therapy
KW - mesenchymal stem cells
KW - neural precursor cells
KW - stem cells
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U2 - 10.1080/02713683.2021.2002909
DO - 10.1080/02713683.2021.2002909
M3 - Article
C2 - 34749546
AN - SCOPUS:85121130733
SN - 0271-3683
VL - 47
SP - 450
EP - 460
JO - Current eye research
JF - Current eye research
IS - 3
ER -