Potential of the ovine brain as a model for anesthesia-induced neuroapoptosis

Olutoyin A. Olutoye*, David A. Lazar, Adesola C. Akinkuotu, Adekunle Adesina, Oluyinka O. Olutoye

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Purpose: This study aimed to determine the feasibility of the fetal ovine model for anesthesia-induced neuroapoptosis detection and effect of dexmedetomidine on neuroapoptosis. Methods: Brains of fetal lambs that underwent tracheal occlusion for congenital diaphragmatic hernia were studied following anesthetic exposure. The brains of nine fetuses from six pregnant sheep were studied. Seven of these fetuses underwent surgery for tracheal balloon insertion at 118–120 days gestational age (GA) under 1.5–2.0 % isoflurane for 2–3 h. Two weeks afterward, at balloon retrieval, a repeat anesthetic: 1.5–2 % isoflurane for 6 h was administered. Five of these fetuses were also exposed to dexmedetomidine concurrently. Immunohistochemistry of fetal brains for apoptotic neurons using activated caspase-3 antibodies was compared to that of an unexposed control group at GA 109 and 122 days. Results: Neuroapoptosis was detected in the ovine fetus with GA- dependent variation observed in the hippocampus. Increased neuroapoptosis occurred in the isoflurane-only group. Fetuses with isoflurane–dexmedetomidine exposure exhibited decreased neuroapoptosis compared to isoflurane-only group. Conclusion: The fetal ovine model is a suitable option for neuroapoptosis analysis. Isoflurane use appears to be associated with additional neuroapoptosis in ovine fetuses undergoing surgical stimulation. Possible amelioration of isoflurane-induced neuroapoptosis by dexmedetomidine deserves further study. Further studies of the effect of gestational age, dose, duration of anesthesia and surgical stimulation on neuroapoptosis are needed.

Original languageEnglish (US)
Pages (from-to)865-869
Number of pages5
JournalPediatric Surgery International
Volume31
Issue number9
DOIs
StatePublished - Sep 20 2015

Keywords

  • Anesthesia
  • Fetal surgery
  • Neuroapoptosis
  • Ovine model

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

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