Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

Chao Qi; Yiwei Tony Zhu; Jeffrey Chang; Anjana V. Yeldandi; M. Sambasiva Rao; Yi Jun Zhu

doi:10.1016/j.bbrc.2004.12.187

Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

Chao Qi, Yiwei Tony Zhu, Jeffrey Chang, Anjana V. Yeldandi, M. Sambasiva Rao, Yi Jun Zhu^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article

25 Scopus citations

Abstract

Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

Original language	English (US)
Pages (from-to)	393-398
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	328
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2004.12.187
State	Published - Mar 11 2005

Keywords

Breast cancer amplified sequence 2
Coactivator
Estrogen receptor α

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2004.12.187

Fingerprint Dive into the research topics of 'Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2'. Together they form a unique fingerprint.

Cite this

Qi, C., Zhu, Y. T., Chang, J., Yeldandi, A. V., Rao, M. S., & Zhu, Y. J. (2005). Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2. Biochemical and Biophysical Research Communications, 328(2), 393-398. https://doi.org/10.1016/j.bbrc.2004.12.187

@article{eeba4a7a22bf4f6892af18502d2af3d5,

title = "Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2",

abstract = "Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.",

keywords = "Breast cancer amplified sequence 2, Coactivator, Estrogen receptor α",

author = "Chao Qi and Zhu, {Yiwei Tony} and Jeffrey Chang and Yeldandi, {Anjana V.} and Rao, {M. Sambasiva} and Zhu, {Yi Jun}",

year = "2005",

month = mar,

day = "11",

doi = "10.1016/j.bbrc.2004.12.187",

language = "English (US)",

volume = "328",

pages = "393--398",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

AU - Qi, Chao

AU - Zhu, Yiwei Tony

AU - Chang, Jeffrey

AU - Yeldandi, Anjana V.

AU - Rao, M. Sambasiva

AU - Zhu, Yi Jun

PY - 2005/3/11

Y1 - 2005/3/11

N2 - Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

AB - Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

KW - Breast cancer amplified sequence 2

KW - Coactivator

KW - Estrogen receptor α

UR - http://www.scopus.com/inward/record.url?scp=13444266088&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13444266088&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2004.12.187

DO - 10.1016/j.bbrc.2004.12.187

M3 - Article

C2 - 15694360

AN - SCOPUS:13444266088

VL - 328

SP - 393

EP - 398

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -