Abstract
Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.
Original language | English (US) |
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Pages (from-to) | 393-398 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 328 |
Issue number | 2 |
DOIs | |
State | Published - Mar 11 2005 |
Keywords
- Breast cancer amplified sequence 2
- Coactivator
- Estrogen receptor α
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology