Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

Chao Qi, Yiwei Tony Zhu, Jeffrey Chang, Anjana V. Yeldandi, M. Sambasiva Rao, Yi Jun Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

Original languageEnglish (US)
Pages (from-to)393-398
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number2
DOIs
StatePublished - Mar 11 2005

Keywords

  • Breast cancer amplified sequence 2
  • Coactivator
  • Estrogen receptor α

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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