Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

Chao Qi; Yiwei Tony Zhu; Jeffrey Chang; Anjana V. Yeldandi; M. Sambasiva Rao; Yi Jun Zhu

doi:10.1016/j.bbrc.2004.12.187

Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

Chao Qi, Yiwei Tony Zhu, Jeffrey Chang, Anjana V. Yeldandi, M. Sambasiva Rao, Yi Jun Zhu^*

^*Corresponding author for this work

Pathology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

Original language	English (US)
Pages (from-to)	393-398
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	328
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2004.12.187
State	Published - Mar 11 2005

Keywords

Breast cancer amplified sequence 2
Coactivator
Estrogen receptor α

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.12.187

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title = "Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2",

abstract = "Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.",

keywords = "Breast cancer amplified sequence 2, Coactivator, Estrogen receptor α",

author = "Chao Qi and Zhu, {Yiwei Tony} and Jeffrey Chang and Yeldandi, {Anjana V.} and Rao, {M. Sambasiva} and Zhu, {Yi Jun}",

note = "Funding Information: We thank Dr. Janardan K. Reddy for his support and comments on the manuscript. We also thank Dr. Yang Shi for plasmid. This work was supported by National Institutes of Health Grants K08 ES 00356 and CA 88898 (To Y.J.Z), and CA 84472 (to M.S.R). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2005",

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doi = "10.1016/j.bbrc.2004.12.187",

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AU - Chang, Jeffrey

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AU - Rao, M. Sambasiva

AU - Zhu, Yi Jun

N1 - Funding Information: We thank Dr. Janardan K. Reddy for his support and comments on the manuscript. We also thank Dr. Yang Shi for plasmid. This work was supported by National Institutes of Health Grants K08 ES 00356 and CA 88898 (To Y.J.Z), and CA 84472 (to M.S.R). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2005/3/11

Y1 - 2005/3/11

N2 - Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

AB - Breast cancer amplified sequence 2 (BCAS2) was initially identified as a gene that was overexpressed and amplified in some breast cancer cell lines. It was later found to be a component of the spliceosome. Here, we identified BCAS2 as an estrogen receptor (ER) α interacting protein by yeast two-hybrid screening. In addition to ERα, BCAS2 also interacted with ERβ, TRβ, PR, and PPARγ in a ligand-independent way. Transient transfection assays revealed that overexpression of BCAS2 enhanced while inhibition of BCAS2 expression attenuated the estrogen receptor-mediated transcription. BCAS2 potentiated the activation function-2 (AF-2) activity of ERα but had no effect on the AF-1 activity. This study suggested that BCAS2 might play an important role in breast cancer development by increasing the estrogen receptor's function.

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Potentiation of estrogen receptor transcriptional activity by breast cancer amplified sequence 2

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