TY - JOUR
T1 - Power spectrum analysis of EEG in a translational nonhuman primate model after chronic exposure to low levels of the common marine neurotoxin, domoic acid
AU - Petroff, R.
AU - Murias, M.
AU - Grant, K. S.
AU - Crouthamel, B.
AU - McKain, N.
AU - Shum, S.
AU - Jing, J.
AU - Isoherranen, N.
AU - Burbacher, T. M.
N1 - Funding Information:
This research was supported by grants from the U.S. National Institutes of Health R01 ES023043 , P51 OD010425 , HD083091 and NCATS Grant TL1 TR000422 (SS).
Funding Information:
This research was supported by grants from the U.S. National Institutes of HealthR01 ES023043, P51 OD010425, HD083091 and NCATS Grant TL1 TR000422 (SS).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/9
Y1 - 2020/9
N2 - Domoic acid (DA), the focus of this research, is a marine algal neurotoxin and epileptogen produced by species in the genus Pseudo-nitzschia. DA is found in finfish and shellfish across the globe. The current regulatory limit for DA consumption (20 ppm in shellfish) was set to protect humans from acute toxic effects, but there is a growing body of evidence suggesting that regular consumption of DA contaminated seafood at or below the regulatory limit may lead to subtle neurological effects in adults. The present research uses a translational nonhuman primate model to assess neurophysiological changes after chronic exposure to DA near the regulatory limit. Sedated electroencephalography (EEG) was used in 20 healthy adult female Macaca fascicularis, orally administered 0.075 and 0.15 mg DA/kg/day for at least 10 months. Paired video and EEG recordings were cleaned and a Fast Fourier Transformation was applied to EEG recordings to assess power differences in frequency bands from 1−20 Hz. When DA exposed animals were compared to controls, power was significantly decreased in the delta band (1−4 Hz, p < 0.005) and significantly increased in the alpha band (5−8 Hz, p < 0.005), theta band (9−12 Hz, p < 0.01), and beta band (13−20 Hz, p < 0.05). The power differences were not dose dependent or related to the duration of DA exposure, or subtle clinical symptoms of DA exposure (intentional tremors). Alterations of power in these bands have been associated with a host of clinical symptoms, such as deficits in memory and neurodegenerative diseases, and ultimately provide new insight into the subclinical toxicity of chronic, low-dose DA exposure on the adult primate brain.
AB - Domoic acid (DA), the focus of this research, is a marine algal neurotoxin and epileptogen produced by species in the genus Pseudo-nitzschia. DA is found in finfish and shellfish across the globe. The current regulatory limit for DA consumption (20 ppm in shellfish) was set to protect humans from acute toxic effects, but there is a growing body of evidence suggesting that regular consumption of DA contaminated seafood at or below the regulatory limit may lead to subtle neurological effects in adults. The present research uses a translational nonhuman primate model to assess neurophysiological changes after chronic exposure to DA near the regulatory limit. Sedated electroencephalography (EEG) was used in 20 healthy adult female Macaca fascicularis, orally administered 0.075 and 0.15 mg DA/kg/day for at least 10 months. Paired video and EEG recordings were cleaned and a Fast Fourier Transformation was applied to EEG recordings to assess power differences in frequency bands from 1−20 Hz. When DA exposed animals were compared to controls, power was significantly decreased in the delta band (1−4 Hz, p < 0.005) and significantly increased in the alpha band (5−8 Hz, p < 0.005), theta band (9−12 Hz, p < 0.01), and beta band (13−20 Hz, p < 0.05). The power differences were not dose dependent or related to the duration of DA exposure, or subtle clinical symptoms of DA exposure (intentional tremors). Alterations of power in these bands have been associated with a host of clinical symptoms, such as deficits in memory and neurodegenerative diseases, and ultimately provide new insight into the subclinical toxicity of chronic, low-dose DA exposure on the adult primate brain.
KW - Chronic exposure
KW - Domoic acid
KW - Electroencephalography
KW - Nonhuman primate
KW - Power spectrum density
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U2 - 10.1016/j.neuro.2020.07.006
DO - 10.1016/j.neuro.2020.07.006
M3 - Article
C2 - 32717199
AN - SCOPUS:85088661222
VL - 80
SP - 124
EP - 129
JO - NeuroToxicology
JF - NeuroToxicology
SN - 0161-813X
ER -