Abstract
We have identified a novel human protein, PRC1, that is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 has sequence homology to the budding yeast anaphase spindle elongation factor Ase1p. Like Ase1p, PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell midbody during cytokinesis. Microinjection of anti-PRC1 antibodies into HeLa cells blocked cellular cleavage, but not nuclear division, indicating a functional role for PRC1 in the process of cytokinesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 877-885 |
| Number of pages | 9 |
| Journal | Molecular cell |
| Volume | 2 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1998 |
Funding
We thank Drs. D. Pellman for the yeast PY1014 ase1 ts strain; J. Massague for His-tagged cyclin E baculovirus; Y. Yao for His-tagged cyclin A baculovirus; D. Chambers for help with FACS analysis; D. A. Peterson and R. Palmer for help with confocal microscopy; T. Pollard and N. Li for critical reading of the manuscript; M. Kanemitsu, L. Potter, and R. Palmer for discussion; and J. Meisenhelder, H. Mondala, and S. Simon for laboratory support. This work was supported by United States Public Health Service grants CA14195 and CA39780 (to T. H.). W. J. was supported by a postdoctoral fellowship from the American Cancer Society. G. J. was supported by a postdoctoral fellowship from NIH. N. J. W. was supported by a postdoctoral fellowship from the Wellcome Trust. T. H. is a Frank and Else Schilling American Cancer Society Research Professor.
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology