Pre-B-cell colony formation assay.

Masato Ikeda*, Richard M Longnecker

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Latent membrane protein 2A (LMP-2A) of Epstein-Barr virus (EBV) mimics a constitutively active B-cell receptor (BCR) and plays a key role in viral latency and EBV pathogenesis. By functioning as a BCR mimic, LMP-2A drives B-cell development, resulting in the bypass of normal B-cell developmental checkpoints. To assess the function of LMP-2A, we have utilized a colony formation assay for the progenitor B cells that uses the B-cell proliferation factor IL-7.

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalMethods in molecular biology (Clifton, N.J.)
Volume292
StatePublished - Jan 1 2005

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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