Precipitation of ACC in liposomes - A model for biomineralization in confined volumes

Chantel C. Tester, Ching Hsuan Wu, Steven Weigand, Derk Joester*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Biomineralizing organisms frequently precipitate minerals in small phospholipid bilayer-delineated compartments. We have established an in vitro model system to investigate the effect of confinement in attoliter to femtoliter volumes on the precipitation of calcium carbonate. In particular, we analyze the growth and stabilization of liposome-encapsulated amorphous calcium carbonate (ACC) nanoparticles using a combination of in situ techniques, cryo-transmission electron microscopy (Cryo-TEM), and small angle X-ray scattering (SAXS). Herein, we discuss ACC nanoparticle growth rate as a function of liposome size, carbon dioxide flux across the liposome membrane, pH, and osmotic pressure. Based on these experiments, we argue that the stabilization of ACC nanoparticles in liposomes is a consequence of a low nucleation rate (high activation barrier) of crystalline polymorphs of calcium carbonate.

Original languageEnglish (US)
Pages (from-to)345-356
Number of pages12
JournalFaraday Discussions
StatePublished - Nov 1 2012

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

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