Precise characterization of human histones in the H2A gene family by top down mass spectrometry

Michael T. Boyne; James J. Pesavento; Craig A. Mizzen; Neil L. Kelleher

doi:10.1021/pr050269n

Precise characterization of human histones in the H2A gene family by top down mass spectrometry

Michael T. Boyne, James J. Pesavento, Craig A. Mizzen, Neil L. Kelleher^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

143 Scopus citations

Abstract

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

Original language	English (US)
Pages (from-to)	248-253
Number of pages	6
Journal	Journal of Proteome Research
Volume	5
Issue number	2
DOIs	https://doi.org/10.1021/pr050269n
State	Published - Feb 2006

Keywords

Cell cycle
Chromatin
Electron capture dissociation (ECD)
Fourier transform mass spectrometry (FTMS)
Histone H2A
Histone code
Post-translational modification (PTM)
SILAC
Top Down
Ubiquitination

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr050269n

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title = "Precise characterization of human histones in the H2A gene family by top down mass spectrometry",

abstract = "Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.",

keywords = "Cell cycle, Chromatin, Electron capture dissociation (ECD), Fourier transform mass spectrometry (FTMS), Histone H2A, Histone code, Post-translational modification (PTM), SILAC, Top Down, Ubiquitination",

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language = "English (US)",

volume = "5",

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journal = "Journal of Proteome Research",

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T1 - Precise characterization of human histones in the H2A gene family by top down mass spectrometry

AU - Boyne, Michael T.

AU - Pesavento, James J.

AU - Mizzen, Craig A.

AU - Kelleher, Neil L.

PY - 2006/2

Y1 - 2006/2

N2 - Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

AB - Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

KW - Cell cycle

KW - Chromatin

KW - Electron capture dissociation (ECD)

KW - Fourier transform mass spectrometry (FTMS)

KW - Histone H2A

KW - Histone code

KW - Post-translational modification (PTM)

KW - SILAC

KW - Top Down

KW - Ubiquitination

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JO - Journal of Proteome Research

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Precise characterization of human histones in the H2A gene family by top down mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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