Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy

Ilya V. Ulasov; Haidn Foster; Mike Butters; Jae Geun Yoon; Tomoko Ozawa; Theodore Nicolaides; Xavier Figueroa; Parvinder Hothi; Michael Prados; John Butters; Charles Cobbs

doi:10.1007/s11060-017-2440-x

Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy

Ilya V. Ulasov^*, Haidn Foster, Mike Butters, Jae Geun Yoon, Tomoko Ozawa, Theodore Nicolaides, Xavier Figueroa, Parvinder Hothi, Michael Prados, John Butters, Charles Cobbs

^*Corresponding author for this work

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30–70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas.

Original language	English (US)
Pages (from-to)	257-264
Number of pages	8
Journal	Journal of Neuro-Oncology
Volume	133
Issue number	2
DOIs	https://doi.org/10.1007/s11060-017-2440-x
State	Published - Jun 1 2017

Keywords

EGFR
Electromagnetic energy
Radio frequency

ASJC Scopus subject areas

Oncology
Neurology
Clinical Neurology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11060-017-2440-x

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title = "Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy",

abstract = "Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30–70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas.",

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author = "Ulasov, {Ilya V.} and Haidn Foster and Mike Butters and Yoon, {Jae Geun} and Tomoko Ozawa and Theodore Nicolaides and Xavier Figueroa and Parvinder Hothi and Michael Prados and John Butters and Charles Cobbs",

note = "Funding Information: We thank all members of IVY Center for Advanced Brain Tumor Treatment at Swedish Neuroscience Institute for useful discussion of experimental approaches and data. This work was carried out through the general support of Center for Advanced Brain Tumor Treatment General Fund, Nativis Inc General Support grant and Swedish Foundation. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media New York.",

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doi = "10.1007/s11060-017-2440-x",

language = "English (US)",

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T1 - Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy

AU - Ulasov, Ilya V.

AU - Foster, Haidn

AU - Butters, Mike

AU - Yoon, Jae Geun

AU - Ozawa, Tomoko

AU - Nicolaides, Theodore

AU - Figueroa, Xavier

AU - Hothi, Parvinder

AU - Prados, Michael

AU - Butters, John

AU - Cobbs, Charles

N1 - Funding Information: We thank all members of IVY Center for Advanced Brain Tumor Treatment at Swedish Neuroscience Institute for useful discussion of experimental approaches and data. This work was carried out through the general support of Center for Advanced Brain Tumor Treatment General Fund, Nativis Inc General Support grant and Swedish Foundation. Publisher Copyright: © 2017, Springer Science+Business Media New York.

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30–70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas.

AB - Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30–70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas.

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KW - Electromagnetic energy

KW - Radio frequency

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Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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